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Characterization of long non-coding RNAs involved in cadmium toxic response in Brassica napus

机译：甘蓝型油菜中镉毒性反应中涉及长期非编码RNA的特征

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There is increasing evidence of long non-coding RNA (lncRNA) involvement in a variety of biological responses to environmental stresses. The toxic heavy metal cadmium (Cd) is one of the major inorganic contaminants in environments. How lncRNAs are involved in Cd stress-induced response and regulatory mechanism in dicot plants is unknown. The present study performed strand-specific RNA-sequencing (RNA-Seq) to profile lncRNAs in Cd-exposed rapeseed ( Brassica napus ). More than 60 million clean reads corresponding to 5038 unique B. napus lncRNAs were identified, in which 2546 lncRNAs were generated from the sense strand and 2492 were generated from natural antisense transcripts (lncNATs). A combinational analysis resulted in identification of 301 lncRNAs responding to Cd stress. Cd-induced expression of lncRNAs appeared to be associated with their nearby protein-coding genes. Many coding genes flanking lncRNA loci were involved in Cd uptake, translocation and stress-responses, suggesting that the associated lncRNAs might have similar functions. Four lncRNAs were identified as precursors of miR824, miR167d, miR156d and miR156e. Sixty-seven lncRNAs acted as competing endogenous target mimics (eTMs) for 36 miRNAs potentially involved in Cd stress response. The functional mimics were validated by transient transformation of three lncRNAs into B. napus protoplasts under control of double 35S promoter with a decoy lncRNA (d35S-lncRNAs-pAN580). These results suggest that a set of putative lncRNAs were involved in response to Cd stress in B. napus .
机译：越来越多的非编码RNA（LNCRNA）参与环境应激的各种生物反应的证据。有毒重金属镉（CD）是环境中主要无机污染物之一。 LNCRNA如何参与CD应激诱导的响应和Dicot植物的调节机制是未知的。本研究表演了STrand特异性的RNA测序（RNA-SEQ），以在CD暴露的油菜籽（Brassica Napus）中的LNCRNA。鉴定了超过6000万个清洁读取，对应于5038个独特的B.NAPUSLNCRNA，其中从感测链中产生2546个LNCRNA，并从自然反义转录物（LNCNATS）产生2492。组合分析导致鉴定301克朗的响应CD胁迫。 LNCRNA的CD诱导的LNCRNA表达似乎与其附近的蛋白质编码基因相关。许多侧翼LNCRNA基因座的编码基因涉及CD吸收，易位和应力响应，表明相关的LNCRNA可能具有类似的功能。将四种LNCRNA鉴定为MIR824，MIR167D，MIR156D和MIR156E的前体。六十七个LNCRNA充当竞争的内源性目标模拟物（ETMS），适用于潜在参与CD应激反应的36 mIRNA。通过诱饵LNCRNA的双35S启动子的控制，通过三个LNCRNA的瞬时转化验证了功能模拟物。用诱饵LNCRNA（D35S-LNCRNA-PAN580）。这些结果表明，响应B. Napus中的CD胁迫参与了一组推定的LNCRNA。

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