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首页> 外文期刊>RSC Advances >Synthesis, characterization, and crystal structure of RNA targeted l- and d-phenylalanine-(1,10-phen)–copper(ii) conjugate complexes: comparative in vitro RNA binding profile of enantiomers and their biological evaluation by morphological studies and antibacterial activity
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Synthesis, characterization, and crystal structure of RNA targeted l- and d-phenylalanine-(1,10-phen)–copper(ii) conjugate complexes: comparative in vitro RNA binding profile of enantiomers and their biological evaluation by morphological studies and antibacterial activity

机译：RNA靶向L-和D-苯丙氨酸 - （1,10-苯甲丙氨酸 - （II）缀合物复合物的合成，表征和晶体结构：对映异构体的比较体外RNA结合谱及其形态学研究的生物学评价及抗菌活性

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New ternary chiral Cu( II ) complexes 1a and b derived from L - and D -phenylalanine and 1,10-phenanthroline were synthesized and characterized thoroughly by single crystal X-ray diffraction and other spectroscopic techniques viz. UV-vis, IR, EPR, ESI-MS and elemental analysis. The complexes crystallized in the monoclinic P 21 space group, possessing the lattice parameters a = 5.74690(10) ?, b = 20.6365(2) ?, c = 9.28010(10) ?, α = γ = 90°, and β = 98.2040(10) in complex 1a and a = 5.728(5) ?, b = 20.587(5) ?, c = 9.252(5) ?, α = γ = 90°, and β = 98.308(5) in complex 1b per unit cell, respectively. Comparative in vitro RNA binding studies of the L - and D -enantiomeric complexes, 1a and b , were carried out by a variety of optical spectroscopy techniques viz. UV-vis, fluorescence, and circular dichroism. Because copper is a redox metal ion, cyclic voltammetry was employed to evaluate the enantioselective RNA binding of the complexes. The results demonstrated that the L -enantiomer of Cu( II ) complex, 1a , binds more strongly to the t-RNA motif than the D -enantiomer, thereby underlining the differential disposition of the enantiomers and the site preference of RNA for the L -enantiomer over the D -enantiomer. Furthermore, the comparative K _(b) , K and K _(sv) values of the L - and D -complexes demonstrated significant increases for the L -enantiomer of the copper complex, 1a , in comparison to its D -enantiomeric form, 1b . SEM analysis divulged surface morphological alteration of complexes 1a and b , evidenced by the formation of hollow tubes and a concrete-like structure with the RNA condensate, which was less pronounced in SEM micrographs of the complex 1b condensate. Complexes 1a and b were evaluated by the agar well diffusion method and demonstrated significant antibacterial activity.
机译：通过单晶X射线衍射和其他光谱技术，通过单晶X射线衍射和其他光谱技术彻底合成新的三元手性Cu（II）复合物1A和B和B衍生自L - 和D-phenylanine和1,10菲咯啉。 UV-Vis，IR，EPR，ESI-MS和元素分析。在单斜斜晶型P 21空间组中结晶的络合物，具有晶格参数A = 5.74690（10）？，B = 20.6365（2）？，C = 9.28010（10）？，α=γ= 90°，β= 98.2040 （10）在复合物1a和a = 5.728（5）？，b = 20.587（5）？，c = 9.252（5）？，α=γ= 90°，每单位复合1b中的β= 98.308（5）细胞分别。通过各种光学光谱技术Viz来进行L - 和D-蒽料复合物，1a和b的比较体外RNA结合研究。 UV-Vis，荧光和圆形二色性。因为铜是氧化还原金属离子，所以使用循环伏安法评估配合物的映射式RNA结合。结果表明，Cu（II）复合物的L-托聚体，1A，比D-蒽酰基更强烈地结合到T-RNA基质，从而强调对映体的差异布置和LNA的位点偏好L - 对致映异构体的映体。此外，L - 和D-复合物的对比k _（b），k和k和k _（sv）值表现出铜络合物的L-致铬聚酯，1a的显着增加，其与其D-蒽形形式相比， 1B。 SEM分析复合物1A和B的表面形态改变，通过形成中空管和具有RNA缩合的混凝土状结构来证明，在络合物1B冷凝物的SEM显微照片中较小。通过琼脂孔扩散方法评估配合物1A和B，并显示出显着的抗菌活性。

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