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首页> 外文期刊>RSC Advances >A T–Hg2+–T metallo-base pair-mediated dual amplification fluorescent strategy for the selective and sensitive detection of Hg2+
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A T–Hg2+–T metallo-base pair-mediated dual amplification fluorescent strategy for the selective and sensitive detection of Hg2+

机译：T-HG2 + -T金属碱基对介导的双扩增荧光策略，用于HG2 +的选择性和敏感性检测

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摘要

The mercuric ion is a highly toxic contaminant and causes severe harm to the environment and human health. Herein, a T–Hg ~(2+) –T metallo-base pair-mediated dual amplification fluorescent strategy was proposed for the selective and sensitive detection of Hg ~(2+) based on a target cycle and DNAzyme cycle. First, Hg ~(2+) selectively bound with T–T mismatches in H-DNA and A-DNA to form stable T–Hg ~(2+) –T metallo-base pairs. This initiated the strand displacement between H-DNA and A-DNA to obtain the Hg ~(2+) -mediated partial double-stranded structure, with a blunt 3′-terminus of the opened H-DNA (donated as the Hg-complex). Next, under the action of Exo III, the Hg-complex was digested to release DNAzyme, A-DNA and Hg ~(2+) . The released Hg ~(2+) could bind with another A-DNA and H-DNA, and the target cycle started anew, eventually generating numerous DNAzymes. DNAzymes then catalyzed the cleavage of a molecular beacon (MB) to generate free fluorophores. Upon cleavage, DNAzymes were released and continuously hybridized with another MB to trigger a second DNAzyme cycle. Finally, numerous fluorophores were liberated, resulting in a significantly amplified signal. The strategy showed a good linear relationship in the range from 2.0 × 10 ~(?10) mol L ~(?1) to 1.0 × 10 ~(?8) mol L ~(?1) , with a detection limit of 7.2 × 10 ~(?11) mol L ~(?1) . The proposed strategy exhibited remarkable selectivity towards Hg ~(2+) against other metal ions. Furthermore, this strategy was successfully applied to detect Hg ~(2+) in real water samples. The proposed strategy provided a reliably quantitative candidate for potential application in environmental monitoring and biotoxicity analysis.
机译：汞离子是一种剧毒污染物，对环境和人类健康造成严重危害。在此，提出了基于靶循环和DNAzyme循环的Hg〜（2+）的选择性和敏感检测的T-Hg〜（2+）-T金属碱基对介导的双扩增荧光策略。首先，Hg〜（2+）用H-DNA和A-DNA中的T-T不匹配选择性地结合，形成稳定的T-Hg〜（2+）-T金属碱对。这引发了H-DNA和A-DNA之间的链位移，得到Hg〜（2+）介导的部分双链结构，其中开放的H-DNA的钝器3'-末端（作为Hg-复合物捐赠）。接下来，在EXO III的作用下，消化了HG-复合物以释放DNAzyme，A-DNA和Hg〜（2+）。释放的Hg〜（2+）可以与另一种A-DNA和H-DNA结合，并且目标循环重新开始，最终产生众多的DNAzymes。然后DNAzymes催化分子信标（MB）的切割以产生游离荧光团。在切割时，释放DNazymes并与另一MB连续杂交以引发第二个DNAzyme循环。最后，释放了许多荧光团，导致了显着放大的信号。该策略在2.0×10〜（α10）摩尔L〜（α1）至1.0×10〜（？8）Mol L〜（α1）的范围内，该策略显示出良好的线性关系，检测限为7.2× 10〜（？11）mol l〜（？1）。所提出的策略对其他金属离子的Hg〜（2+）表现出显着的选择性。此外，该策略成功地应用于检测真实水样中的Hg〜（2+）。拟议的策略为环境监测和生物毒性分析提供了可靠的定量候选人。

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