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首页> 外文期刊>RSC Advances >Design of a core–shell support to improve lipase features by immobilization
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Design of a core–shell support to improve lipase features by immobilization

机译：核心壳支持的设计，通过固定改善脂肪酶特征

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Different core–shell polymeric supports, exhibiting different morphologies and composition, were produced through simultaneous suspension and emulsion polymerization, using styrene (S) and divinylbenzene (DVB) as co-monomers. Supports composed of polystyrene in both the core and the shell (PS/PS) and the new poly(styrene- co -divinylbenzene) support (PS- co -DVB/PS- co -DVB) were used for the immobilization of three different lipases (from Rhizomucor miehie (RML), from Themomyces lanuginosus (TLL) and the form B from Candida antarctica , (CALB)) and of the phospholipase Lecitase Ultra (LU). The features of the new biocatalysts were evaluated and compared to the properties of commercial biocatalysts (Novozym 435 (CALB), Lipozyme RM IM and Lipozyme TL IM) and biocatalysts prepared by enzyme immobilization onto commercial octyl-agarose, a support reported as very suitable for lipase immobilization. It was shown that protein loading and stability of the biocatalysts prepared with the core–shell supports were higher than the ones obtained with commercial octyl-agarose or the commercial lipase preparations. Besides, it was shown that the biocatalysts prepared with the core–shell supports also presented higher activities than commercial biocatalysts when employing different substrates, encouraging the use of the produced core–shell supports for immobilization of lipases and the development of new applications.
机译：通过同时悬浮液和乳液聚合，使用苯乙烯（S）和二乙烯基苯（DVB）作为共聚单体，通过同时悬浮液和乳液聚合制备不同的核壳聚合物载体，其表现出不同的形态和组合物。在核和壳（PS / PS）和新的聚（苯乙烯-co-二乙烯基苯）的支持（PS-共-DVB / PS-共-DVB）两者聚苯乙烯组成的载体被用于三个不同的脂肪酶固定化（从根茎中（RML），从HOMOMYCESLANUGINOSS（TLL）和来自Candida抗野原的形式B，（CALB））和磷脂酶合唱酶Ultra（Lu）。评估新生物催眠催化剂的特征，并与商业生物催化剂的性质（Novozym 435（CALB），Lipozyme RM IM和Lipozyme TL IM）和通过酶固定化制备的生物催化剂进行了比较，所述酶固定到商业辛基 - 琼脂糖上，称为非常适合的载体脂肪酶固定化。结果表明，用核 - 壳载体制备的生物催化剂的蛋白质负载和稳定性高于商业辛基 - 琼脂糖或商业脂肪酶制剂所获得的蛋白质负载和稳定性。此外，结果表明，在采用不同的基材时，用核心 - 壳体支撑件制备的生物催化剂也比商业生物催化剂呈现更高的活动，促进所生产的核心壳支撑件用于固定脂肪酶和新应用的开发。

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