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首页> 外文期刊>RSC Advances >Fabrication and characterization of ALK1fc-loaded fluoro-magnetic nanoparticles for inhibiting TGF β1 in hepatocellular carcinoma
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Fabrication and characterization of ALK1fc-loaded fluoro-magnetic nanoparticles for inhibiting TGF β1 in hepatocellular carcinoma

机译：抑制肝细胞癌TGFβ1的ALK1FC氟磁性纳米粒子的制备与表征

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Fluoro-magnetic nanoparticles play an important role in biomedical applications since their size and concentration in tumors allow a very high resolution and an accurate mapping of lesions. Fluorescein isothiocyanate (FITC) has been entrapped inside crystals of magnetic nanoparticles (MNPs) during crystallization. This causes changes of nanoparticle crystal architecture towards elongated rods. TEM and SEM-EDX show elongated crystals with high iron concentration. The intensity of fluoro-MNP fluorescence was detected by fluorescence spectrophotometry and confocal microscopy. The benzene ring structure of FITC and its carboxylic group were clearly detected in the fluoro-MNP spectrum by using FTIR, compared to MNPs prepared in the absence of FITC. Rods were functionalized by hydrogel cross-linking structure (PEG-CMC) onto the fluoro-MNPs surface by using alternate layer-by-layer (LbL) adsorption. These hydrogel properties are used as a preserver for protein delivery. ALK1fc as specific TGFβ1 inhibitor, was encapsulated inside (PEG-CMC) layers during LbL assembly. Zeta potential measurement, X-ray diffraction and SDS PAGE-silver staining results confirmed the encapsulation of ALK1fc. The efficiency of encapsulated ALK1fc was quantified by immunofluorescence assay against localization of TGFβ1. Stained TGFβ1 appeared a purple color and is distributed in the cytoplasm of untreated HLF (a liver cancer invasive cell line), whereas it disappeared in a HLF sample treated with encapsulated ALK1fc.
机译：氟 - 磁性纳米颗粒在生物医学应用中起重要作用，因为肿瘤中的大小和浓度允许非常高的分辨率和精确的病变映射。荧光素异硫氰酸酯（FITC）在结晶期间被夹在磁性纳米颗粒（MNP）的晶体中。这导致纳米粒子晶体架构朝向细长杆的变化。 TEM和SEM-EDX显示具有高铁浓度的细长晶体。通过荧光分光光度法和共聚焦显微镜检测氟-MNP荧光的强度。通过使用FTIR在氟-mNP光谱中清楚地检测FITC及其羧基的苯环结构，与在没有FITC的情况下制备的MNP相比，在氟-MNP光谱中清楚地检测到氟-MNP光谱中。通过使用替代的逐层（LBL）吸附，通过水凝胶交联结构（PEG-CMC）通过水凝胶交联结构（PEG-CMC）官能化棒。这些水凝胶属性用作蛋白质递送的浆液。作为特异性TGFβ1抑制剂的ALK1FC被包封在LBL组件期间的内部（PEG-CMC）层。 Zeta电位测量，X射线衍射和SDS页面银染色结果证实了ALK1FC的包封。通过免疫荧光测定对TGFβ1的定位量化封装的ALK1FC的效率。染色的TGFβ1出现了紫色，并分布在未处理的HLF（肝癌侵入性细胞系）的细胞质中，而它在用包封的ALK1FC处理的HLF样品中消失。

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