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Immobilization of horseradish peroxidase enzymes on hydrous-titanium and application for phenol removal

机译：固定辣根过氧化物酶对含水 - 钛的磷酸化酶及苯酚去除效应

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In this work, hydrous titanium was utilized to immobilize horseradish peroxidase (HRP) in order to improve its stability and adaptability under different water qualities using a biomimetic titanification process. The catalytic performance of immobilized HRP for phenol removal from aqueous solution was evaluated. The physicochemical properties of immobilized HRP (IM-HRP) were characterized by means of scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FT-IR). The effects of the reaction conditions (H _(2) O _(2) and IM-HRP dosages and initial phenol concentration) on phenol removal were investigated. Enzymatic activity analysis indicated that the immobilization process has no adverse effects on the catalytic performance of HRP, and a slight increase in the enzymatic catalytic kinetic constant ( k _(m) ) of IM-HRP was observed compared to its free enzymes. It was found that at a synthetic temperature of 37 °C ± 3 °C, IM-HRP exhibited excellent phenol removal of over 90% after reaction for 15 min. In addition, the enzyme activity of IM-HRP was very stable over a wide range of reaction conditions ( e.g. , temperatures from 20 °C to 90 °C and pH from 3.0 to 11.0). Therefore, HRP enzymes loaded on hydrous titanium are very effective at improving the stability of enzymes and can alleviate the adverse effects of various environmental and water conditions on contaminant removal.
机译：在这项工作中，利用含水钛固定辣根过氧化物酶（HRP），以便使用仿生钛化方法改善不同水质的稳定性和适应性。评价了固定的HRP的催化性能，用于从水溶液中除去水溶液。通过扫描电子显微镜（SEM），透射电子显微镜（TEM）和傅里叶变换红外光谱（FT-IR），表征固定化HRP（IM-HRP）的物理化学性质。研究了反应条件（H _（2）O _（2）和IM-HRP剂量和初始酚浓度）对苯酚去除的影响。酶活性分析表明，固定过程对HRP的催化性能没有不利影响，与其游离酶相比，观察到IM-HRP的酶催化动力学常数（K _（M））的略微增加。发现在37℃±3℃的合成温度下，在反应15分钟后，IM-HRP在反应后出现优异的苯酚除去超过90％。此外，IM-HRP的酶活性在宽范围的反应条件下非常稳定（例如，从20℃至90℃的温度和3.0至11.0的pH）。因此，在含水钛上装载的HRP酶在提高酶的稳定性方面非常有效，并且可以减轻各种环境和水条件对污染物去除的不利影响。

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