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首页> 外文期刊>RSC Advances >A sensitive electrogenerated chemiluminescence biosensor for galactosyltransferase activity analysis based on a graphitic carbon nitride nanosheet interface and polystyrene microsphere-enhanced responses
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A sensitive electrogenerated chemiluminescence biosensor for galactosyltransferase activity analysis based on a graphitic carbon nitride nanosheet interface and polystyrene microsphere-enhanced responses

机译：基于石墨氮化碳含氮纳米片界面和聚苯乙烯微球增强响应的敏感电化学化学发光生物传感器

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In this manuscript, a sandwich electrogenerated chemiluminescence (ECL) biosensor for galactosyltransferase (Gal T) activity analysis based on a graphitic carbon nitride (g-C _(3) N _(4) ) nanosheet interface and polystyrene microsphere-enhanced responses is described. The carboxylated g-C _(3) N _(4) nanosheet was firstly coated on the GC electrode surface, and then the bovine serum albumin conjugated with N -acetylglucosamine (GlcNAc-BSA) was further modified on the g-C _(3) N _(4) modified GC electrode. In the presence of Gal T and UDP-Gal as a coreactor, galactose was conjugated to GlcNAc-BSA, and the ECL signal of the GlcNAc-BSA conjugated g-C _(3) N _(4) nanosheet modified electrode decreased. After the adsorption of Artocarpus integrifolia lectin (AIA) conjugated polystyrene microsphere (PSM) nanoprobes through the specific interaction between galactose and AIA, the ECL signal decreased significantly due to the poor conductivity of the nanoprobes, which inhibited the electron transfer on the electrode interface, leading to high sensitivity toward Gal T activity analysis. The detection limit of 7 × 10 ~(?5) U mL ~(?1) was obtained. The proposed ECL sensor has been applied to Gal T activity analysis in serum samples as well as Gal T activity expression in different cell lines, and may be a novel tool to for glycosyltransferase activity evaluation and inhibition in clinic diagnostics. This strategy can also be extended to detect and monitor other glycosyltransferases.
机译：在该稿件中，描述了基于石墨酰基转移酶（GAL T）活性分析的夹层电流化学发光（ECL）生物传感器（G-C _（3）N _（4））纳米液界面和聚苯乙烯微球增强的响应。首先涂覆在GC电极表面上的羧化的GC _（3）N _（4）纳米片，然后在GC _（3）N _上进一步修饰与N-乙酰葡糖胺（GLCNAC-BSA）缀合的牛血清白蛋白（4）改进的GC电极。在GAL T和UDP-GAL作为对反应器的存在下，半乳糖与GLCNAC-BSA缀合，并且GLCNAC-BSA缀合的G-C_（3）N _（4）N _（4）纳米片改性电极的ECL信号降低。通过半乳糖和AIA之间的特异性相互作用吸附Artocarpus entegifolia凝集凝集素（AIA）缀合的聚苯乙烯微球（PSM）纳米素，由于纳米素的导电性差，ECL信号显着降低，这抑制了电极接口上的电子转移，导致高敏感性对GAL T活动分析。获得7×10〜（α5）U ml〜（α1）的检出限。所提出的ECL传感器已应用于血清样品中的GAL T活性分析以及不同细胞系中的GAL T活性表达，并且可以是用于糖基转移酶活性评价和临床诊断中抑制的新型工具。该策略也可以扩展以检测和监测其他糖基转移酶。

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