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Electrochemical detection of two tumor markers based on functionalized polypyrrole microspheres as immunoprobes

机译：基于官能化聚吡咯微球的两种肿瘤标志物的电化学检测

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In this study, a sandwich-type electrochemical immunosensor for the simultaneous detection of two tumor markers has been reported using functionalized polypyrrole microspheres as immunoprobes. The polypyrrole microspheres, which were synthesized through an one step chemical oxidative polymerization approach, showed high adsorption ability to redox probes such as thionine and adriamycin. Meanwhile, the polypyrrole microspheres functionalized with redox probes and gold nanoparticles (Au NPs) provided a larger surface area for the two different antibodies immobilization. The reduced graphene oxide sheet (rGO) was decorated with Au NPs as a substrate to immobilize two different antibodies. Carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) were used as model analytes in this study. Two well resolved reduction peaks, one at about ?0.26 V (corresponding to thionine), another at about ?0.69 V (corresponding to adriamycin, both vs. SCE) can be obtained in differential pulse voltammograms. Under optimal conditions, CEA and AFP can be simultaneously detected in the range from 1.0 pg mL ~(?1) to 50 ng mL ~(?1) . The limit of detection for CEA and AFP was 0.40 pg mL ~(?1) and 0.33 pg mL ~(?1) (at S/N = 3), respectively. Further, the immunosensor was evaluated using a human serum sample, and the recovery was within 99.5–107% for CEA and 96.3–99.9% for AFP. The analysis results with the serum sample using the immunosensor were in accordance with those of an enzyme linked immunosorbent assay (ELISA), indicating the immunosensor has a potential application in real sample analysis.
机译：在该研究中，使用官能化的聚吡咯微球同时检测用于同时检测两种肿瘤标志物的夹层型电化学免疫传感器。通过一种阶梯化学氧化聚合方法合成的聚吡咯微球，表明氧化还原探针如硫胺和亚霉素等高吸附能力。同时，用氧化还原探针和金纳米颗粒（Au nps）官能化的聚吡咯微球提供了两种不同抗体固定的较大表面积。将还原的石墨烯氧化物片（RGO）用Au nps作为底物装饰，以固定两种不同的抗体。癌胚抗原（CEA）和α-胎蛋白（AFP）用作本研究中的模型分析物。两个良好的解决峰值，一个在约0.26V（对应于噻吩）的情况下，在差分脉冲伏安图中可以获得关于0.69V（对应于Adriamycin的adriamycin）。在最佳条件下，CEA和AFP可以在1.0pg ml〜（α1）至50ng ml〜（α1）的范围内同时检测。 CEA和AFP的检测极限分别为0.40pg ml〜（α1）和0.33pg ml〜（α1）（在s / n = 3）。此外，使用人血清样品评价免疫传感器，回收率在CEA的99.5-107％内，AFP的96.3-99.9％。使用免疫传感器的血清样品的分析结果符合酶联免疫吸附测定（ELISA）的样品，表明免疫传感器具有实际样品分析中的潜在应用。

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