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Enhanced binding capacity of boronate affinity fibrous material for effective enrichment of nucleosides in urine samples

机译：增强硼酸盐亲和纤维材料的结合能力，以有效富集尿液中的核苷

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In this work, polyethyleneimine-modified boronate affinity fibrous cotton with good selectivity and higher binding capacity for cis -diols was developed for in-pipette-tip solid-phase extraction (SPE). The introduction of polyethyleneimine for the modification of cotton fiber provided abundant binding sites for adsorption of cis -diols, resulting in higher binding capacities up to 300 μg g ~(?1) for catechol, 450 μg g ~(?1) for dopamine and 700 μg g ~(?1) for adenosine, respectively. The adsorbent could still show recognition ability towards cis -diols even with a 1000-fold amount of interferences in the solution. Several extraction parameters were optimized, including adsorbent dose, pipette times, washing and elution solvents. Under optimal extraction conditions, the proposed in-pipette-tip SPE method coupled with reversed-phase liquid chromatography was successfully used to extract nucleosides in urine. The limit of detection was 3.5–4.7 ng mL ~(?1) and the recoveries were in the range of 89–102% with relative standard deviations of less than 9.9%. In conclusion, combination of the specific adsorption performance of the prepared boronate affinity fibrous material and in-pipette-tip SPE provided a fast pretreatment method of trace cis -diols in complex real samples.
机译：在这项工作中，为管滴尖端固相萃取（SPE）开发了具有良好选择性和更高的CIS-二醇的聚乙烯亚胺改性的硼酸硼亲和力纤维棉。用于改性棉纤维的聚乙烯亚胺提供了丰富的结合位点，用于吸附Cis -diols，导致儿茶酚的高达300μgg〜（β1）的结合能力，450μgg〜（α1）用于多巴胺和腺苷的700μgg〜（α1）分别用于腺苷。吸附剂仍然可以显示CIS-二醇的识别能力，即使在溶液中有1000倍的干扰量。优化了几种提取参数，包括吸附剂量，移液管时间，洗涤和洗脱溶剂。在最佳提取条件下，提出的引发的移液管尖端SPE方法与反相液相色谱相结合，成功地用于提取尿液中的核苷。检测的极限为3.5-4.7 ng ml〜（α1），回收率在89-102％的范围内，相对标准偏差小于9.9％。总之，制备的硼酸硼亲和力纤维材料和移液管尖端SPE的特异性吸附性能的组合提供了一种快速预处理的痕量CIS二醇在复杂的真实样品中。

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