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首页> 外文期刊>RSC Advances >Fluorescent yeast containing intracellularly biosynthesized CdSe QDs as a sensitive probe for simple determination of copper(ii) in water and plasma
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Fluorescent yeast containing intracellularly biosynthesized CdSe QDs as a sensitive probe for simple determination of copper(ii) in water and plasma

机译：含有细胞内生物合成的CDSE QDS的荧光酵母作为敏感探针，用于在水和血浆中简单测定铜（II）

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A simple strategy using CdSe QD-containing yeast as a fluorescent probe was developed for tracing copper( II ) (Cu ~(2+) ) in water and plasma. Nanocrystal CdSe QDs were attentively biosynthesized by Saccharomyces cerevisiae in vivo and were well reserved in the cytoplasm. The yeast membrane, with selective permeability, acted as a semipermeable filter to protect the intrafungal QDs from external interference, for example, from certain ions, peptides, proteins and insoluble solids. Moreover, the as-produced CdSe QDs were capped with cell-derived peptides and proteins, ruling out additional intracellular interference from the cytoplasm, including from some ions, lipids and nucleic acids. With the combination of the dual selective effects of both the yeast membrane and the capping proteins of the QDs, this methodology offered a rapid and reliable method for Cu ~(2+) detection in water and plasma with detection limits as low as 1 μmol L ~(?1) and 2 μmol L ~(?1) , respectively. In addition, the presence of the chelating reagent CN ~(?) could selectively chelate Cu ~(2+) ions into Cu(CN) _( n ) ~(( n ?2)? a ) , resulting in the fluorescence recovery of the QDs, which further reinforced the specificity of Cu ~(2+) detection. These results suggest that this technique via biogenic QD-containing microbes provided great potential for body fluid Cu ~(2+) determination with superior selectivity, reliability and simplicity.
机译：为追踪铜（II）（Cu〜（2+））在水和血浆中开发了使用Cdse QD含酵母作为荧光探针的简单策略。纳米晶CdSe QDS通过体内酿酒酵母酿酒酵母术分泌物化，并在细胞质中保留很好。具有选择性渗透性的酵母膜用作半透滤器，以保护肠QD免受外部干扰，例如来自某些离子，肽，蛋白质和不溶性固体。此外，用细胞衍生的肽和蛋白质封装了各种CDSE QD，从细胞质中排出额外的细胞内干扰，包括来自一些离子，脂质和核酸。随着酵母膜的双重选择性效果的组合和QDS的封端蛋白，该方法提供了一种在水和等离子体中的Cu〜（2+）检测的快速可靠的方法，具有低至1μmolL的检测限〜（？1）和2μmol1〜（？1）。另外，螯合剂CN〜（α）的存在可以选择性地将Cu〜（2+）离子螯合成Cu（CN）_（N）〜（（N 2）→a），导致荧光恢复QDS进一步加强Cu〜（2+）检测的特异性。这些结果表明，该技术通过含生物QD的微生物提供了具有优异选择性，可靠性和简单性的体液Cu〜（2+）测定的巨大潜力。

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