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首页> 外文期刊>RSC Advances >PEI–folic acid modified carbon nanodots for cancer cell-targeted delivery and two-photon excitation imaging
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PEI–folic acid modified carbon nanodots for cancer cell-targeted delivery and two-photon excitation imaging

机译：Pei-叶酸改性碳纳米蛋白癌细胞靶向递送和双光子励磁成像

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摘要

Polyethyleneimine conjugated folic acid (PEI–FA) was coated onto the surfaces of fluorescent carbon nanodots (CDots) under neutral conditions through electrostatic interactions between the partially charged amino groups of PEI–FA and the carboxyl (–COOH) groups of the CDot surfaces. Because of the high abundance of folate receptors (FR) in cancer cells, folic acid (FA) was used as the targeting ligand to enhance the CDots' binding capability and penetration into the target cancer cells. We evaluated the amount of particles internalized by cancer cell lines displaying various levels of folate receptors. Two-photon excitation microscopy images revealed that the uptake of CDot–PEI–FA by FR-positive KB cancer cells was much higher than that by FR-negative A549 cancer cells. Moreover, quantitative flow cytometry analysis confirmed the receptor-mediated targeted delivery of the nanoparticles into different FR-expressing cells. The internalization of particles was ten-fold higher in the FR-positive KB cancer cells compared to that in the FR-negative A549 cancer cells; the difference was significant enough to be of biological importance. These preliminary results demonstrate the promising potential of CDot–PEI–FA for FR-positive cancer cell line diagnosis.
机译：将聚乙烯亚胺缀合的叶酸（PEI-FA）涂覆到中性条件下的荧光碳纳多（Cdots）的表面上，通过CDot表面的部分带电氨基和羧基（-COOH）基团的部分带电氨基之间的静电相互作用。由于癌细胞中叶酸受体（FR）的高丰度，叶酸（Fa）用作靶向配体，以增强Cdots的结合能力和渗透到靶癌细胞中。我们评估了显示各种叶酸受体的癌细胞系内部化的颗粒量。双光子激发显微镜图像显示Cdot-Pei-Fa通过FR阳性Kb癌细胞的摄取远高于FR阴性A549癌细胞的摄取。此外，定量流式细胞术分析证实了纳米颗粒的受体介导的靶向递送到不同的FR表达细胞中。与FR阴性A549癌细胞相比，FR阳性KB癌细胞中颗粒的内化在FR阳性KB癌细胞中较高。差异足以具有生物重要性。这些初步结果证明了CDOT-PEI-FA用于FR阳性癌细胞系诊断的有希望的潜力。

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