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The cadmium binding characteristics of a lactic acid bacterium in aqueous solutions and its application for removal of cadmium from fruit and vegetable juices

机译:水溶液中乳酸菌的镉结合特征及其去除水果和蔬菜汁中镉的应用

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Heavy metal cadmium (Cd) is an environmental pollutant that causes adverse health effects in humans. This toxic metal has been detected in a wide range of fruit and vegetables. A strain of lactic acid bacteria, Lactobacillus plantarum CCFM8610, was screened out for its good ability to bind Cd, and this study was designed to investigate the Cd binding properties of this bacterium, and to evaluate its use for removal of Cd from fruit and vegetable juices. Electron microscopy observations and energy dispersive X-ray analysis confirmed that the majority of the Cd was bound to the surface of the bacterial cell. The Cd biosorption of L. plantarum CCFM8610 was strongly pH dependent, and carboxyl and amino groups of the bacterial surface molecules are important in the binding process. The biosorption was fast and efficient, and could be well explained by the Langmuir–Freundlich dual isotherm model ( R ~(2) = 0.99) and the pseudo second-order kinetic model ( R ~(2) = 0.99). After a 2 h incubation and a simple centrifugation, L. plantarum CCFM8610 treatment removed 67% to 82% of the Cd from nine types of fruit and vegetable juices. Long-period fermentation by L. plantarum CCFM8610 (36 h) also significantly decreased Cd concentrations in the juices (56% to 81%). Our results show that this food-grade bacterial strain could be used as a potential probiotic for Cd removal from fruit and vegetable juices.
机译:重金属镉(CD)是一种环境污染物,导致人类的不良反应。这种有毒的金属已在各种水果和蔬菜中检测到。筛选乳酸菌乳酸菌乳酸菌乳酸杆菌菌株,筛选出良好的结合CD的能力,并且该研究旨在研究该细菌的CD结合性能,并评估其用于从水果和蔬菜中去除CD的用途果汁。电子显微镜观察和能量分散X射线分析证实,大部分CD与细菌细胞表面结合。 L.Portararum CCFM8610的Cd生物吸附依赖性强,细菌表面分子的羧基和氨基在结合过程中是重要的。生物吸附快速有效,可以通过Langmuir-Freundlich双等温模型(R〜(2)= 0.99)和伪二阶动力学模型(R〜(2)= 0.99)很好。孵育2小时后,L.Purerarum CCFM8610治疗从九种水果和蔬菜汁中除去67%至82%的CD。 L.Portararum CCFM8610(36H)的长期发酵也显着降低了果汁中的Cd浓度(56%至81%)。我们的研究结果表明,这种食品级细菌菌株可用作从水果和蔬菜汁中去除CD的潜在益生菌。

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