• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Methodology for quantitative determination of the carbohydrate composition of brown seaweeds (Laminariaceae)
【24h】

Methodology for quantitative determination of the carbohydrate composition of brown seaweeds (Laminariaceae)

机译：用于定量测定棕色海藻（Laminariaceae）碳水化合物组成的方法

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The monosaccharide composition of four different samples of brown seaweeds Laminaria digitata and Saccharina latissima were compared by different high performance anion exchange chromatography (HPAEC) methods after different acid hydrolysis treatments or a cellulase treatment. A two-step treatment of 72% (w/w) H _(2) SO _(4) + 4% (w/w) H _(2) SO _(4) performed best, but cellulase treatment released more glucose than acid treatments. HPAEC with pulsed amperometric detection (PAD) allowed quantification of all present neutral sugars and the sugar alcohol mannitol. Furthermore, the use of guluronic, glucuronic, and galacturonic acid as standards enabled quantification of the uronic acids. A complete map of amino acids, fatty compounds, minerals, and ash was also achieved. L. digitata and S. latissima harvested in Denmark April (Baltic Sea, 2012) were dominated by alginic acid and ash (each ～30% by weight (w/w) of the dry matter) and 10% (w/w) protein. In contrast, the dominant compound of L. digitata harvested in August (North Sea, 2012) was glucose constituting 51% w/w of the dry matter, and with 16% w/w alginic acid. Washing prior to analysis mainly removed salts.
机译：通过不同的酸性水解处理或纤维素酶治疗后，通过不同的高性能阴离子交换色谱（HPAEC）方法比较四种不同样品的棕色海藻棕榈藻Digitata和Saccharina Latissima的单糖组合物。两步处理72％（w / w）h _（2）所以_（4）+ 4％（w / w）h _（2）所以_（4）表现最佳，但纤维素酶治疗释放出更多的葡萄糖而不是酸治疗。 HPAEC具有脉冲安培检测（PAD）允许所有现有中性糖和糖醇甘露醇的定量定量。此外，使用吞咽，葡萄糖和半乳糖醛酸作为标准的标准，使得核酸酸的定量。还实现了氨基酸，脂肪化合物，矿物质和灰分的完整图。 L. Digitata和S. Latissima在丹麦4月（波罗的海，2012年）由海藻酸和灰（每〜30重量％（W / W）的干物质）和10％（w / w）蛋白质主导。相比之下，8月份的L. Digitata的主要化合物（北海，2012年）是葡萄糖构成51％w / w的干物质，16％w / w海藻酸。在分析之前洗涤主要取下盐。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号