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Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP

机译:阿特拉嗪降解细菌拟菌涂层的长期活性涂料.Pseudomonas sp。 ADP.

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The atrazine-degrading bacterial strain Pseudomonas sp. ADP was immobilized by the sol–gel process within thin silica layers coated onto water-retaining carrier materials (expanded clay pellets and scoria). The performance of the obtained biohybrid material has been investigated concerning long-term activity under non-growth conditions. Experiments were run in phosphate buffer containing atrazine (20 mg l ~(?1) ) as the sole source of carbon and nitrogen. Even after one year of consecutive batch tests, P. ADP immobilized onto expanded clay pellets showed a high atrazine degradation activity. In the course of long-term batch experiments, the average amount of removed atrazine was about 94% during each assay cycle. Staining with CTC revealed that in spite of cultivation under non-growth conditions over a period of one year, immobilized cells were still vital and showed respiratory activity.
机译:阿特拉津降解细菌菌株假鼠SP。通过涂覆在水保持载体材料(膨胀的粘土颗粒和焦化)上的薄二氧化硅层内的溶胶 - 凝胶法固定ADP。已经在非生长条件下研究了所得生物冬小材料的性能。在含有阿特拉嗪的磷酸盐缓冲液中运行实验(20mg L〜(α1))作为碳和氮的唯一来源。即使连续一年的分批测试后,P. ADP固定在膨胀粘土颗粒上显示出高尿嘧啶降解活性。在长期批量实验过程中,在每个测定循环期间,除去的阿特拉嗪的平均量约为94%。用CTC染色显示,尽管在一年内的非生长条件下培养,但固定化细胞仍然至关重要并显示出呼吸活性。

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