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首页> 外文期刊>Scientific reports. >Effects of multiple injections on the efficacy and cytotoxicity of folate-targeted magnetite nanoparticles as theranostic agents for MRI detection and magnetic hyperthermia therapy of tumor cells
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Effects of multiple injections on the efficacy and cytotoxicity of folate-targeted magnetite nanoparticles as theranostic agents for MRI detection and magnetic hyperthermia therapy of tumor cells

机译：多次注射对叶酸靶向磁铁矿纳米粒子的疗效和细胞毒性作为肿瘤细胞MRI检测和磁体热疗治疗的疗效和细胞毒性

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Folate-targeted iron oxide nanoparticles (FA@Fesub3/subOsub4/sub NPs) were prepared by a one-pot hydrothermal method and then used as cancer theranostic agents by combining magnetic resonance imaging (MRI) and magnetic hyperthermia therapy (MHT). Crystal structure, morphology, magnetic properties, surface functional group, and heating efficacy of the synthesized nanoparticles were characterized by XRD, TEM, VSM, FTIR, and hyperthermia analyses. The results indicated that the crystal structure, magnetic properties, and heating efficacy of the magnetite nanoparticles were improved by hydrothermal treatment. Toxicity of the prepared NPs was assessed in vitro and in vivo on the mammary cells and BALB/c mice, respectively. The results of the in vitro toxicity analysis showed that the FA@Fesub3/subOsub4/sub NPs are relatively safe even at high concentrations of the NPs up to 1000?μg?mLsup-1/sup. Also, the targetability of the FA@Fesub3/subOsub4/sub NPs for the detection of folate over-expressed cancer cells was evaluated in an animal model of breast tumor using MRI analysis. It was observed that Tsub2/sub-weighted magnetic resonance signal intensity was decreased with the three-time injection of the FA@Fesub3/subOsub4/sub NPs with 24?h interval at a safe dose (50?mg?kgsup-1/sup), indicating the accumulation and retention of the NPs within the tumor tissues. Moreover, the therapeutic efficacy of the MHT using the FA@Fesub3/subOsub4/sub NPs was evaluated in vivo in breast tumor-bearing mice. Hyperthermia treatment was carried out under a safe alternating magnetic field permissible for magnetic hyperthermia treatment (f?=?150?kHz, H?=?12.5 mT). The therapeutic effects of the MHT were evaluated by monitoring the tumor volume during the treatment period. The results showed that the mice in the control group experienced an almost 3.5-fold increase in the tumor volume during 15 days, while, the mice in the MHT group had a mild increase in the tumor volume (1.8-fold) within the same period (P??0.05). These outcomes give promise that FA@Fesub3/subOsub4/sub NPs can be used as theranostic agents for the MRI and MHT applications.
机译：叶酸氧化铁纳米颗粒（FA @ Fe 3 O 4 nps）由单锅水热法制备，然后通过组合磁共振成像用作癌症治疗剂（MRI）和磁体热疗治疗（MHT）。通过XRD，TEM，VSM，FTIR和高温分析表征了合成纳米颗粒的晶体结构，形态，磁性，表面官能团和加热功效。结果表明，通过水热处理改善了磁铁矿纳米颗粒的晶体结构，磁性和加热功效。在乳腺细胞和BALB / C小鼠中，在体外和体内评估制备的NPS的毒性。体外毒性分析的结果表明，即使在高浓度为1000Ω×ml 3 o 4 nps也相对安全。 sup> -1 。而且，使用MRI分析，在乳腺肿瘤的动物模型中评估FA @ Fe 3 o 4 nps的靶向甲状腺肿的癌细胞。观察到，T 2 - 重量磁共振信号强度随着FA @ FE 3 O 4 NPS的三次注射而降低在安全剂量（50Ω·kg -1-sup>）处，24Ω·h间隔，表明肿瘤组织内的NP积累和保留。此外，MHT使用FA @ Fe 3 O 4 nps的治疗效果被在乳腺瘤小鼠体内评价。在允许磁体热疗处理的安全交替磁场下进行热疗治疗（F？= 150 kHz，H？= 12.5吨）。通过在治疗期间监测肿瘤体积来评估MHT的治疗效果。结果表明，对照组的小鼠在15天内经历了肿瘤体积的差异几乎增加了3.5倍，而MHT组的小鼠在同一时期内的肿瘤体积（1.8倍）的温和增加（p？<？0.05）。这些结果给出了Fa @ Fe 3 O 4 nps可用作MRI和MHT应用的Theranostic代理。

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