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首页> 外文期刊>Scientific reports. >Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters
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Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters

机译：用超顺磁性氧化铁纳米粒子标记的脐带干细胞磁共振成像：标记和移植参数的影响

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Cell tracking with magnetic resonance imaging (MRI) is important for evaluating the biodistribution of transplanted cells. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have emerged as a promising therapeutic tool in regenerative medicine. We examined the UC-MSCs labeled with superparamagnetic (SPIO) and ultrasmall superparamagnetic iron oxide (USPIO) in terms of cell functioning and imaging efficiency in vitro and in vivo. The UC-MSCs were co-incubated with SPIO or USPIO at a concentration of 50 or 100?μg/mL of label. Viability and proliferation were assessed by Trypan blue dye exclusion and MTT assay, respectively. Differentiation (chondrogenesis, osteogenesis, and adipogenesis) was induced to examine the impact of labelling on stemness. For in vitro experiments, we used 7-T MRI to assess the T2 values of phantoms containing various concentrations of cell suspensions. For in vivo experiments, nine neonatal rats were divided into the control, SPIO, and USPIO groups. The UC-MSCs were injected directly into the rat brains. MRI images were obtained immediately and at 7 and 14?days post injection. The UC-MSCs were successfully labeled with SPIO and USPIO after 24?h of incubation. Cell viability was not changed by labelling. Nevertheless, labelling with 100?μg/mL USPIO led to a significant decrease in proliferation. The capacity for differentiation into cartilage was influenced by 100?μg/mL of SPIO. MRI showed that labeled cells exhibited clear hypointense signals, unlike unlabeled control cells. In the USPIO-labeled cells, a significant (P??0.05) decrease in T2 values (=?improved contrast) was observed when compared with the controls and between phantoms containing the fewest and the most cells (0.5?×?106 versus 2.0?×?106 cells/mL). In vivo, the labeled cells were discernible on T2-weighted images at days 0, 7, and 14. The presence of SPIO and USPIO particles at day 14 was confirmed by Prussian blue staining. Microscopy also suggested that the regions occupied by the particles were not as large as the corresponding hypointense areas observed on MRI. Both labels were readily taken up by the UC-MSCs and identified well on MRI. While SPIO and USPIO provide improved results in MRI studies, care must be taken while labelling cells with high concentrations of these agents.
机译：具有磁共振成像（MRI）的电池跟踪对于评估移植细胞的生物分布是重要的。脐带衍生的间充质干细胞（UC-MSCs）作为再生医学的有希望的治疗工具出现。我们在体外和体内进行了细胞功能和成像效率方面，检查了用超顺磁性（SPIO）和超显硅氧化铁（USPIO）标记的UC-MSCs。将UC-MSC与SPOO或USPIO共孵育，浓度为50或100μg/ ml标记。通过锥虫蓝染料排除和MTT测定评估活力和增殖。诱导分化（软骨发生，骨化和脂肪发生）来检查标记对茎的影响。对于体外实验，我们使用7-T MRI来评估含有各种浓度的细胞悬浮液的幽灵的T2值。对于体内实验，九只新生大鼠分为对照，斯凯赛和USPIO组。将UC-MSC直接注射到大脑脑中。 MRI图像立即获得，并在7和14日何时注射后。在孵化24℃后，UC-MSCs成功标记为SPIO和USPIO。通过标记不会改变细胞活力。然而，用100μg/ ml USPIO标记导致增殖的显着降低。将软骨分化为100μg/ ml的硅氧化物的能力。与未标记的对照细胞不同，MRI表明标记的细胞表现出透明的低音信号。在USPIO标记的细胞中，在与对照组相比和含有最少细胞和最多细胞的幽灵之间的T2值（=α<β05）的显着（p≤0.05）降低，并且在含有最少的细胞和最多的细胞（0.5？×106与2.0？×106个细胞/ ml）。在体内，在第0,7和14天的T2加权图像上可辨别标记的细胞。通过普鲁士蓝染色确认了第14天的硅藻和USPIO颗粒的存在。显微镜也表明颗粒占据的区域并不像MRI上观察到的相应低迷区域那么大。 UC-MSCs易于占用两个标签，并在MRI上识别出良好。虽然SPIO和USPIO在MRI研究中提供了改善的结果，但必须在具有高浓度的这些药剂的标记细胞时进行护理。

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