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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Detection of Toxoplasma gondii by Loop-mediated Isothermal Amplification in Blood and Urine Samples from Women, Saudi Arabia
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Detection of Toxoplasma gondii by Loop-mediated Isothermal Amplification in Blood and Urine Samples from Women, Saudi Arabia

机译：通过来自女性的血液和尿液样本的环介导的等温扩增的毒素弓形虫的检测

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Toxoplasmosis is the disease which is caused by the protozoan parasite Toxoplasma gondii (T. gondii ), which has the ability to infect all warm-blooded animals. Several molecular techniques for the diagnosis of Toxoplasma gondii includes normal and Real Time Polymerase Chain Reaction (RT-PCR), and gene sequencing.Aim: To apply Loop-mediated Isothermal Amplification (LAMP) for the diagnosis of T. gondii infections in urine and blood samples from female population in Saudi Arabia.Materials and Methods: In this cross-sectional study, genomic DNA was extracted from 22 blood and urine samples (11 each), two LAMP assays based on B1 and Surface Antigen 2 (SAG2) genes of T. gondii was performed. Conventional PCR was done for the LAMP product followed by sequencing to confirm the specificity of LAMP method. Statistical Package for the Social Sciences (SPSS) version 20.0 was used to summarise continuous and categorical variables.Results: From 22 samples, 17 samples were LAMP positive in both urine and blood samples, four were negative in blood and positive in urine samples, and one was positive in blood and negative in urine. Sequencing of PCR product confirm the specificity of the method used.Conclusion: LAMP detection of T. gondii DNA is an appropriate, sensitive and specific method for diagnosis of toxoplasmosis from urine and blood specimens in humans.
机译：弓形虫病是由原生动物寄生虫引起的疾病弓形虫（ T.Gondii）引起的，这具有感染所有温血动物的能力。用于诊断的几种分子技术弓形弓形虫包括正常和实时聚合酶链反应（RT-PCR）和基因测序。目的：施加环介导的等温扩增（灯）以诊断<我> t。来自沙特阿拉伯的女性人口的尿液和血液样本中的脑电图感染。材料和方法：在这种横断面研究中，基因组DNA从22例血液和尿液样品中提取（11各自），基于B1的两个灯测定 T的表面抗原2（SAG2）基因。演出了Gondii。常规PCR为灯产品进行，然后进行测序以确认灯法的特异性。社会科学版（SPSS）版本20.0的统计包用于总结连续和分类变量。结果：从22个样品中，尿液和血液样本中的17个样品阳性阳性，血液中的四个是阴性，尿液样品，一个在尿液中呈阳性和阴性。 PCR产物的测序证实了所用方法的特异性。结论：灯检测 t。 Gondii DNA是一种适当，敏感的和特异性的方法，用于诊断人类尿液和血液标本的弓形虫病。

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