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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Efficacy of Different Reagents to Prepare Transparent Tooth Model for Three Dimensional View of the Root Canal Systems- An In-vitro Study
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Efficacy of Different Reagents to Prepare Transparent Tooth Model for Three Dimensional View of the Root Canal Systems- An In-vitro Study

机译：不同试剂为根管系统三维视图制备透明齿模型的疗效 - 体外研究

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There are certain common as well as atypical characteristics in the root canal anatomy of each tooth. Simultaneous understanding of both can be the key to successful endodontics. Various methods have been used till date for visualisation of root canal morphology with variable success rates like magnification, radiography, CBCT, digital imaging, clearing. Clearing technique provides a three-dimensional view of the root canal anatomy with the exterior while maintaining the original form and relationship of canals as minimal instrumentation is required in the procedure. It is a simple and inexpensive technique which involves decalcification of tooth structure, dehydration in alcohol and clearing by immersing in clearing agents.Aim: This study aimed to evaluate the efficacy of two decalcifying agents (nitric acid and trichloroacetic acid) and two clearing agents (methyl salicylate and eugenol) in the preparation of transparent tooth model for viewing the root canal systems.Materials and Methods: The in-vitro research study from August 2, 2019 to August 27, 2019 included forty freshly extracted teeth (18 maxillary and 22 mandibular) which were randomly divided in two groups (n=20); group A (decalcification done in 5% nitric acid) and group B (decalcification done in 10% trichloroacetic acid). Then, the teeth were dehydrated in isopropyl alcohol. Each group was subdivided in two subgroups depending on the clearing agent used (methyl salicylate or eugenol). Afterwards they were graded for transparency and haziness criteria, and data analysis was done using chi-square test value of significance at p<0.05.Results: The results showed that nitric acid took lesser time for decalcification than trichloroacetic acid. The subgroup 1 (NA/MS) showed better transparency level in 90% of the samples when compared with other subgroups; however the results were not significant with p-value 0.121. Haziness was present in all samples. The subgroup 4 (TCA/E) showed 100% haziness which was statistically significant among the all subgroups with p-value=0.002. Methyl salicylate showed better transparency, less haziness and good root canal morphology when compared with eugenol.Conclusion: Nitric acid when used in combination with methyl salicylate prepared the best transparent tooth model. Trichloroacetic acid showed average transparency and haziness was present in almost all the samples irrespective of the clearing agents used.
机译：每个牙齿的根管解剖结构中存在某些常见的和非典型特征。同时理解两者都可以成为成功牙髓的关键。迄今已使用各种方法，以便具有变量成功率的根管形态可视化，如放大率，射线照相，CBCT，数字成像，清除。清除技术提供了与外部的根管解剖结构的三维视图，同时保持原有的形状和作为在过程中需要最小仪器的原始形式和关系。这是一种简单且廉价的技术，涉及牙齿结构的脱钙，醇中的脱水，通过浸入清热剂中清除。目的：本研究旨在评估两个脱钙剂（硝酸和三氯乙酸）的功效和两个在制备用于观察根管系统的透明牙齿模型中的清除剂（甲基水杨酸甲酯和丁香酚）。材料与方法：2019年8月2日至2019年8月27日的体外研究研究包括四十鲜明的牙齿（18个上颌和22个颌骨），其随机分为两组（n = 20）; A组（在5％硝酸中完成的脱钙）和B组（在10％三氯乙酸中完成脱钙）。然后，牙齿在异丙醇中脱水。根据使用的清除剂（水杨酸甲酯或丁香醇），每组细分各组。之后，他们被评分为透明度和饱和标准，并且使用Chi-Square测试值在P <0.05时使用Chi-Square试验值进行了数据分析。结果：结果表明，硝酸比三氯乙酸的脱钙时间较少。与其他亚组相比，亚组1（NA / MS）在90％的样品中显示出更好的透明度水平;然而，P值为0.121，结果不显着。所有样品中存在危险。亚组4（TCA / e）显示出100％的危险，在所有亚组中具有p值= 0.002的亚组之间存在统计学意义。与丁醇相比，甲基水杨酸甲酯显示出更好的透明度，较少的浑浊和良好的根管形态。结论：与水杨酸甲酯组合使用时，硝酸制备了最佳透明齿模型。三氯乙酸显示出平均透明性，并且在几乎所有样品中存在危险，而不管使用的清除剂。

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