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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Utility of Lemon Solution Rehydration Technique for Processing Haemorrhagic Cytology Samples: A Comparative Study using Different Techniques
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Utility of Lemon Solution Rehydration Technique for Processing Haemorrhagic Cytology Samples: A Comparative Study using Different Techniques

机译：柠檬溶液再水合技术用于处理出血性细胞学样本的效用：使用不同技术的比较研究

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Haemorrhagic fluids samples are commonly received for cytological evaluation. The presence of erythrocytes obscures the morphology of cells and thus poses great diagnostic difficulty. Haemorrhagic body fluids are processed by using a variety of techniques. The idea of each technique is to select and concentrate an adequate number of tumor cells having intact cell morphologies, without losing the diagnostically important cells during processing.Aim: To evaluate the effect of known haemolysing solutions [Carnoy’s Fixative (CF) and normal saline {Normal Saline Rehydration Technique (NSRT)}] and 10% lemon solution {Lemon Solution Rehydration Technique (LSRT)} on haemorrhagic cytology samples; and to compare various cytomorphological features in haemorrhagic cytology samples processed by different haemolysing solutions.Materials and Methods: It was an analytical study done at a rural tertiary care referral institute, PES Institute of Medical Sciences and Research (PESIMSR), Kuppam, Andhra Pradesh, India. Fifty-seven haemorrhagic samples were analysed. For each case, totally eight smears were prepared. Out of eight smears, two smears served as control (not treated with any haemolysing agent. Remaining six smears (two smears each) were treated with haemolysing agents (CF, normal saline and 10% lemon solution).Smears stained with Haematoxylin and Eosin (HandE) and May-Grunwald Geimsa (MGG) stains were evaluated for haemolysis in the smear background, retention of cells and cytomorphological details in comparison with control smears. Each smear were scored (1-4) according to a modified scoring system. Chi-square test was the statistical tool used to analyse the data in the study.Results: MGG stained smears showed best haemolysing effect with NSRT (78.95%), maximum retention with LSRT (85.96%) and best cytomorphological details with CF (12.28%). HandEstained smears showed best haemolytic effect with LSRT (56.14%), maximum retention with LSRT (87.72%) and best cytomorphological details with CF (8.77%).Conclusion: LSRT is a novel rehydration technique developed to improve the quality of processing haemorrhagic cytology samples. LSRT showed best overall performance and it may be utilised to overcome the disadvantages of CF and NSRT.
机译：夸张的液体样品通常接受细胞学评估。红细胞的存在模糊了细胞的形态，从而造成了很大的诊断困难。通过使用各种技术处理出血性体液。每种技术的思想是选择并浓缩具有完整细胞形态的足够数量的肿瘤细胞，而不会在加工过程中丢失诊断重要的细胞。目的：评估已知的氧化溶液[Carnoy的固定剂（CF）和正常盐水{正常盐水再水合技术（NSRT）}]和10％柠檬溶液{柠檬溶液再水解技术（LSRT）}在出血性细胞学样本上;并比较由不同氧化溶液加工的出血性细胞学样品中的各种细胞形态特征。材料和方法：它是在农村第三节护理研究所，PES医学科学研究所（Pesimsr），Kuppam的分析研究，安得拉邦，印度。分析了五十七种出血样品。对于每种情况，准备完全八种涂片。在八个涂片中，两种涂片用作控制（未用任何氧化剂治疗。剩余的六种涂片（每种涂片）用氧化剂（CF，正常盐水和10％柠檬溶液）处理。用Haemoxylin和eosin染色（手工）和May-Grunwald Geimsa（MGG）污渍在涂片背景下进行溶血，保留细胞和细胞形态细节与对照涂片相比。根据改性评分系统，评分每个涂片（1-4）。Chi-方形测试是用于分析研究中的数据的统计工具。结果：MGG染色涂片显示出最佳的NSRT（78.95％），最大保留LSRT（85.96％）和最佳细胞骨髓细节，CF（12.28 ％）。令人着迷的涂片显示出最佳的溶血效果，LSRT（56.14％），LSRT（87.72％）最大限度地保持（87.72％）和最佳细胞素细节，CF（8.77％）。结论：LSRT是一种新的再水合技术，以改善质量处理出血性细胞学样本。 LSRT显示出最佳整体性能，可用于克服CF和​​NSRT的缺点。

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