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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Cytomegalovirus Infection Monitoring in Bone Marrow Transplant Recipients from Whole Blood versus Plasma using Real-Time PCR
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Cytomegalovirus Infection Monitoring in Bone Marrow Transplant Recipients from Whole Blood versus Plasma using Real-Time PCR

机译：使用实时PCR，来自全血与血浆的骨髓移植受者在骨髓移植受者中进行塞细胞病毒感染监测

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Cytomegalovirus (CMV) infection in immune compromised bone marrow transplant recipients is known. Its infection is about 90% in Indian population, but its symptoms in various age groups in different blood components are scanty and variable.Aim: To evaluate the Whole Blood (WB) and Plasma (PL) samples in population of adult and paediatric leukaemia bone marrow recipients using real-time PCR for CMV infection monitoring.Materials and Methods: In present observational study, total of 1021 allogeneic bone marrow transplant recipients from our referral centre, including 90 adults and 171 children (paediatric) with follow-up cases were analysed for viral loads in all cases. A suitable RT PCR assigned to amplify the conserved region of the UL132 gene to study with respect to two biological components (WLandPL) was investigated in all the patients.Results: The results for CMV infection analysed on both PL and WB showed a good correlation. The kinetics of CMV DNA viral load within the same patient in both WB and PL showed no significant difference. In 2112 follow-up low level of CMV DNA persisted in WB (4.78%), while it was absent in PL.Conclusion: The use of CMV PCR for WB and PL is appropriate to guide implementation and discontinuation of therapy to CMV might lower the risk of CMV disease when given accurate treatment. It might also assist reduce the side effects and duration of treatment. Detection of residual viremia in WB and PL might also offer comparable results in our study cohort.
机译：已知巨细胞病毒（CMV）感染在免疫损伤的骨髓移植受体中。印度人群的感染约为90％，但其不同血液成分中各种年龄组的症状是稀少和变量的。目的：评估成人人群中的全血（WB）和血浆（PL）样本和儿科白血病骨髓受体使用实时PCR用于CMV感染监测。材料和方法：目前观察研究，来自推荐中心的1021个同种异体骨髓移植受者，其中包括90名成人和171名儿童（儿科）在所有情况下分析病毒载体的随访病例。在所有患者中研究了分配给扩增UL132基因的保守区域的合适RT PCR在所有患者中都研究了研究。结果：在PL和WB上分析CMV感染的结果显示A.良好的相关性。在WB和PL的同一患者中CMV DNA病毒载体的动力学显示出没有显着差异。在2112中，在Wb（4.78％）中持续存在的后续低水平的CMV DNA（4.78％），而在PL中不存在。结论：使用CMV PCR用于WB和PL，适用于指导实施和停止治疗治疗与CMV在给定准确处理时可能降低CMV疾病的风险。它还可以帮助降低副作用和治疗持续时间。 WB和PL中的残留病毒血症检测也可能在我们的研究队列中提供可比的结果。

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