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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Underfilled K2EDTA Vacutainer on Automated Haematological Blood Cell Indices- To Reject or Reconsider?
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Underfilled K2EDTA Vacutainer on Automated Haematological Blood Cell Indices- To Reject or Reconsider?

机译：底层K2EDTA VACUTAINER在自动烘干血液细胞指标上 - 拒绝或重新考虑？

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The most common reason for rejection of blood samples in laboratory is because they are under-filled. Clinical and Laboratory Standards Institute (CLSI) guidelines state that blood vacutainers that contain either 10% higher or lower blood than the recommended volume must be rejected. Repeated phlebotomy has to be performed for such samples which can delay the turnaround time.Aim: To study the effects of underfilled K2EDTA vacutainers on automated haematology indices and compare them with the standard.Materials and Methods: This observational and comparative study was conducted at the department of pathology, Chettinad Hospital and Research Institute, Tamil Nadu for a duration of 6 months from January 2019 to June 2019. A 100 paired samples of underfilled K2EDTA vacutainers were compared with the standard for Complete Blood Count (CBC) analysis using BECKMAN COULTER LH 780 analyser. Statistical analyses was done using Statistical Package for Social Science (SPSS) software v.18 and paired studenta??s t-test. The p-value <0.05 was considered statistically significant.Results: All the parameters in the CBC showed comparable results between under-filled K2EDTA vacutainers of 1 mL blood volume and standard 3 mL blood volume vacutainers. There was no significant variation in statistics even when the blood volume is as low as 1 mL which is 67% less than that of the recommended volume.Conclusion: Blood samples which are under-filled upto 67% less than recommended volume, i.e. upto 1 ml can be accepted for CBC analysis using BECKMAN COULTER LH 780 analyser. Therefore, the CBC data obtained from the under-filled vacutainers can be used for clinical diagnosis in situations where repeat blood collection is challenging or impossible.
机译：在实验室中抑制血液样品的最常见原因是因为它们被填满。临床和实验室标准研究所（CLSI）指导指南说明含有10％或更低的血液的血液空腔必须拒绝。必须对可以延迟周转时间的样本进行重复的噬菌体术。目的：研究底层的K2EDTA Vacutainers对自动血液指数的影响，并将它们与标准进行比较。材料和方法：这种观察和方法比较研究在泰米尔纳德纳德州病理学，切尔丁德医院和研究所，从2019年1月至2019年6月持续了6个月。将100个底层K2EDTA芳土的成对样品与完全血统计数的标准进行比较（CBC ）使用Beckman Coulter LH 780分析仪进行分析。统计分析是使用社会科学（SPSS）软件v.18的统计包进行统计分析，并配对学生的T检验。 P值<0.05被认为是统计学意义。结果：CBC中的所有参数显示出1ml血容量1 ml血量和标准3ml血液体积真空剂的填充内的K2EDTA芳腔之间的相当结果。统计学中没有显着的变化，即使血液体积低至1mL比推荐体积小67％。结论：血液样品填充的血液样本高达67％，比推荐体积低67％，使用Beckman Coulter LH 780分析仪可以接受CBC分析的高达1mL。因此，从填充填充填充血液收集挑战或不可能的情况下可以用于临床诊断的CBC数据可用于临床诊断。

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