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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Use of Ertapenem as a Marker for Detection of Carbapenem Resistance for Enterobacteriaceae
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Use of Ertapenem as a Marker for Detection of Carbapenem Resistance for Enterobacteriaceae

机译：使用ertapeNem作为用于检测肠杆菌植物的Carbapenem抵抗的标记

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Detection of carbapenem resistance in the clinical microbiology laboratory is challenging. Production of carbapenemase enzymes remains the most important mechanism among Carbapenem Resistant Enterobacteriaceae (CRE). Ertapenem has been found as a sensitive marker for detecting CRE, especially the non-carbapenemase producing CRE. However, limited literature is available discussing its specificity and sensitivity in comparison to gold standard tests.Aim: To compare the ability of the ertapenem disc diffusion test with other confirmatory tests i.e., Epsilometer test (E test), Carbapenemase Nordmann-Poirel (CNP) test, and Polymerase Chain Reaction (PCR) for CRE identification.Materials and Methods: Seventy six phenotypically confirmed Enterobacteriaceae isolates were tested for carbapenem resistance. Ertapenem susceptibility was compared with imipenem, meropenem, and doripenem disc individually and in combination to determine its sensitivity. Further, it was compared with the E test, CNP test, and PCR to find the concordance of the result. Data were analysed by statistical software using Chisquare test with p-value <0.05 as significant.Results: Ertapenem disc independently was able to detect maximum resistant isolates (64/76) in comparison to other individual carbapenem discs or their combinations. Among the four carbapenem discs, the result of the ertapenem disc showed maximum concordance with its corresponding E test. The sensitivity, specificity, Positive Predictive Value (PPV), and Negative Predictive Value (NPV) of the Ertapenem disc compared to the gold standard tests (CNP and PCR) were 89.7%, 62.5%, 95.3%, and 41.7%, respectively.Conclusion: Disc diffusion test using ertapenem disc was observed as a sensitive marker for detecting CRE. The result of the ertapenem disc diffusion test was observed less discordant with E test, CNP test, and PCR in comparison to other carbapenem discs.
机译：检测临床微生物实验室在临床微生物学实验室中的抗性。碳结构酶的产量仍然是Carbapenem耐药肠杆菌（CRE）中最重要的机制。已经发现ERTAPENEM作为检测CRE的敏感标志物，尤其是产生克雷的非碳结构酶。然而，与金标准测试相比，有限的文献讨论其特异性和敏感性。目的：比较EARTapeNEM椎间盘扩散试验与其他确认测试的能力，即肺活度计测试（E测试），Carbapenemase Nordmann-Poirel （CNP）试验和CRE鉴定的聚合酶链反应（PCR）。材料和方法：测试七十六种表型证实的肠杆菌区分离株，用于肉豆胺抗性。与ImipeNem，Meropenem和Doripenem圆盘单独且组合以确定其灵敏度的比较。此外，将其与E试验，CNP测试和PCR进行比较，以找到结果的一致性。通过使用Chisquare测试使用P值<0.05的统计软件进行分析数据。结果：与其他单独的碳蛋白圆盘或其组合相比，ErtapeNem光盘独立能够检测最大抗性隔离物（64/76）。在四个鲤鱼椎间盘中，ERTAPENEM椎间盘的结果与其相应的E测试显示出最大的一致性。与黄金标准试验（CNP和PCR）相比，ERTAPENEM圆盘的敏感性，特异性，阳性预测值（PPV）和阴性预测值（NPV）分别为89.7％，62.5％，95.3％和41.7％。 结论：使用ERTAPENEM椎间盘盘的光盘扩散测试作为检测CRE的敏感标记。与其他CarbapeNem圆盘相比，观察到近赤蛋白椎间盘扩散试验的结果与E试验，CNP测试和PCR相比。

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