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首页> 外文期刊>Scientific reports. >Increased flux in acetyl-CoA synthetic pathway and TCA cycle of Kluyveromyces marxianus under respiratory conditions
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Increased flux in acetyl-CoA synthetic pathway and TCA cycle of Kluyveromyces marxianus under respiratory conditions

机译：在呼吸状况下，乙酰CoA合成途径和TCA循环中的乙酰-Co合成途径和TCA循环增加

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摘要

Yeasts are extremely useful, not only for fermentation but also for a wide spectrum of fuel and chemical productions. We analyzed the overall metabolic turnover and transcript dynamics in glycolysis and the TCA cycle, revealing the difference in adaptive pyruvate metabolic response between a Crabtree-negative species, Kluyveromyces marxianus, and a Crabtree-positive species, Saccharomyces cerevisiae, during aerobic growth. Pyruvate metabolism was inclined toward ethanol production under aerobic conditions in S. cerevisiae, while increased transcript abundances of the genes involved in ethanol metabolism and those encoding pyruvate dehydrogenase were seen in K. marxianus, indicating the augmentation of acetyl-CoA synthesis. Furthermore, different metabolic turnover in the TCA cycle was observed in the two species: malate and fumarate production in S. cerevisiae was higher than in K. marxianus, irrespective of aeration; however, fluxes of both the reductive and oxidative TCA cycles were enhanced in K. marxianus by aeration, implying both the cycles contribute to efficient electron flux without producing ethanol. Additionally, decreased hexokinase activity under aerobic conditions is expected to be important for maintenance of suitable carbon flux. These findings demonstrate differences in the key metabolic trait of yeasts employing respiration or fermentation, and provide important insight into the metabolic engineering of yeasts.
机译：酵母非常有用，不仅用于发酵，而且对于广谱的燃料和化学产品也是如此。我们分析了糖酵解和TCA循环中的整体代谢营业额和转录性动态，揭示了Crabtree阴性物种，Kluyveromyces Marxianus和Crabtree阳性物种，酿酒酵母的适应性丙酮酸代谢反应的差异在有氧生长期间。丙酮酸代谢在酿酒酵母的好氧条件下倾向于乙醇产量，而在K.Marxianus中观察到参与乙醇代谢和编码丙酮酸脱氢酶的基因的转录物丰富。此外，在两种物种中观察到TCA循环中的不同代谢营业额：酿酒酵母在酿酒酵母中的较大的比K.Marxianus，无论曝气都高于K. Marxianus;然而，通过通气，在K.Marxianus中增强了还原性和氧化TCA循环的助熔剂，暗示循环均有助于有效的电子通量而不产生乙醇。另外，预期有氧条件下的六酮酶活性降低对维持合适的碳通量是重要的。这些研究结果表明了使用呼吸或发酵的酵母关键代谢性状的差异，并对酵母的代谢工程提供重要的洞察。

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