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首页> 外文期刊>Scientific reports. >Ultrasound delivery of Surface Enhanced InfraRed Absorption active gold-nanoprobes into fibroblast cells: a biological study via Synchrotron-based InfraRed microanalysis at single cell level
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Ultrasound delivery of Surface Enhanced InfraRed Absorption active gold-nanoprobes into fibroblast cells: a biological study via Synchrotron-based InfraRed microanalysis at single cell level

机译：将表面增强的红外吸收活性金纳米体释放到成纤维细胞中的超声递送：通过单细胞水平的同步rotron的红外微分分析生物学研究

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Ultrasound (US) induced transient membrane permeabilisation has emerged as a hugely promising tool for the delivery of exogenous vectors through the cytoplasmic membrane, paving the way to the design of novel anticancer strategies by targeting functional nanomaterials to specific biological sites. An essential step towards this end is the detailed recognition of suitably marked nanoparticles in sonoporated cells and the investigation of the potential related biological effects. By taking advantage of Synchrotron Radiation Fourier Transform Infrared micro-spectroscopy (SR-microFTIR) in providing highly sensitive analysis at the single cell level, we studied the internalisation of a nanoprobe within fibroblasts (NIH-3T3) promoted by low-intensity US. To this aim we employed 20?nm gold nanoparticles conjugated with the IR marker 4-aminothiophenol. The significant Surface Enhanced Infrared Absorption provided by the nanoprobes, with an absorbance increase up to two orders of magnitude, allowed us to efficiently recognise their inclusion within cells. Notably, the selective and stable SR-microFTIR detection from single cells that have internalised the nanoprobe exhibited clear changes in both shape and intensity of the spectral profile, highlighting the occurrence of biological effects. Flow cytometry, immunofluorescence and murine cytokinesis-block micronucleus assays confirmed the presence of slight but significant cytotoxic and genotoxic events associated with the US-nanoprobe combined treatments. Our results can provide novel hints towards US and nanomedicine combined strategies for cell spectral imaging as well as drug delivery-based therapies.
机译：超声（US）诱导的瞬时膜透透析是通过细胞质膜递送外源性载体的一种强大有前途的工具，通过针对特定的生物位点靶向新的抗癌策略的设计。朝向该目的的重要步骤是在声波潜气细胞中对适当标记的纳米颗粒的详细识别以及对潜在的相关生物学效应进行调查。通过利用同步辐射傅里叶变换红外微谱（SR-MicroDir）在单细胞水平提供高度敏感的分析时，我们研究了通过低强度US促进的成纤维细胞（NIH-3T3）内的纳米孔内的内化。对于此目的，我们使用20？NM金纳米颗粒与IR标记4-氨基酚苯酚缀合。由纳米素提供的显着表面增强的红外吸收，吸光度增加到两个数量级，使我们能够有效地识别它们在细胞内的夹杂物。值得注意的是，从具有内化纳米孔的单个细胞的选择性和稳定的SR-MicroDir检测表现出光谱曲线的形状和强度的透明变化，突出了生物效应的发生。流式细胞术，免疫荧光和鼠细胞因子嵌段微核微核测定证实存在与US-Nanoprobe组合处理相关的轻微但显着的细胞毒性和遗传毒性事件。我们的结果可以为我们和纳米医生的组合策略提供新颖的细胞光谱成像以及药物递送的疗法。

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