The surface area is an important parameter in setting any biorefining technology. The aim of this study was to investigate the applicability of sorption of methylene blue to characterize the surface of the main biomass carbohydrates: α-cellulose, sigmacell cellulose, natural gum, β-glucan, and starch. The morphology of particles of the model objects was studied by scanning electron microscopy. Nitrogen adsorption isotherms demonstrate that the selected carbohydrates are macroporous adsorbents. The monolayer capacities, the energy constants of the Brunauer–Emmett–Teller (BET) equation, and specific surface areas were calculated using the BET theory, the comparative method proposed by Gregg and Sing, and the Harkins–Jura method. The method of methylene blue sorption onto biomass carbohydrates was adapted and mastered. It was demonstrated that sorption of methylene blue proceeds successfully in ethanol, thus facilitating surface characterization for carbohydrates that are either soluble in water or regain water. It was found that the methylene blue sorption values correlate with specific surface area determined by nitrogen adsorption/desorption and calculated from the granulometric data. As a result of electrostatic attraction, the presence of ion-exchanged groups on the analyte surface has a stronger effect on binding of methylene blue than the surface area does. Sorption of methylene blue can be used in addition to gas adsorption/desorption to assess the accessibility of carbohydrate surface for binding large molecules.
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