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外文期刊>Applied Ecology and Environmental Research
>PHYSIOLOGICAL AND PHYTOCHEMICAL RESPONSES OF BABY SPINACH (SPINACIA OLERACEA L.) CULTIVARS TO COMBINED NPKS NUTRITION AND BACILLUS SUBTILIS BD233 INOCULATION USING LC-MS
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PHYSIOLOGICAL AND PHYTOCHEMICAL RESPONSES OF BABY SPINACH (SPINACIA OLERACEA L.) CULTIVARS TO COMBINED NPKS NUTRITION AND BACILLUS SUBTILIS BD233 INOCULATION USING LC-MS
Baby spinach (Spinacia oleracea L.) is grown for its nutritional benefits, edible leaves and medicinal purpose. The use of crop amendments such as inoculation with plant growth-promoting bacteria (PGPB) together with fertilizers for crop cultivation is more sustainable as it reduces the excessive use of fertilizers and increases crop yield. The aim of this study was to evaluate the physiological and phytochemical response of baby spinach cultivars to different levels of Nitrogen, Phosphorus, Potassium and Sulphur (NPKS) nutrition, amended with Bacillus subtilis strain BD233 inoculation. A factorial field experiment with treatments arranged in a randomized complete block design (RCBD) was carried out. The results showed that NPKS fertilization significantly affected the total biomass of baby spinach but not B. subtilis (BD233) inoculation and cultivar or their interaction. The level of chlorophyll varied between cultivars ranging from 19.96±1.62b mg/m2 and 24.79±1.98a mg/m2 , however, no significant differences occurred in stomatal conductance among the three cultivars. However, significant difference was observed on compounds among cultivars with NPKS fertilizer application in most identified compounds such as patuletin-3-glucosyl-(1-6)[apiosyl(1-2)]-glucoside, spinacetin-3-glucosyl-(1-6)[apiosyl(1-2)]-glucoside, (S)-Malate and N-Acetyl-D-tryptophan. Therefore, the study recommends that fertilizer at 22N:22P:30K:5S kg/ha and 33N:33P:45K:7S kg/ha amended with B. subtilis inoculation be considered when cultivating baby spinach.
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机译：婴儿菠菜（Spinacia Oleracea L.）以其营养益处，食用叶和药用目的而生长。使用植物生长细菌（PGPB）和作物培养的肥料中的作物修正的使用更加可持续，因为它减少了过度使用肥料并增加了作物产量。本研究的目的是评估婴儿菠菜品种对不同水平的氮，磷，钾和硫（NPKS）营养水平的生理和植物化学响应，用枯草芽孢杆菌菌株BD233接种修正。进行了在随机完整块设计（RCBD）中排列的治疗的阶乘领域实验。结果表明，NPKS施肥显着影响了婴儿菠菜的总生物量，但不是B.枯草芽孢杆菌（BD233）接种和品种或其相互作用。然而，叶绿素之间的水平范围从19.96±1.62b mg / m 2和24.79±1.98a mg / m 2之间发生巨大差异在三种品种的气孔导率下没有显着差异。然而，在大多数鉴定的化合物如髌粉蛋白-3-葡糖基 - （1-6）中的含有NPKS肥料施用中的含有NPKs肥料施用的化合物上观察到显着差异。（1-2） - 葡糖苷，Sparacetin-3-葡糖基 - （1 -6）[阿甲酸酯（1-2）] - 葡糖苷，（s） - alate和n-acetyl-d-色氨酸。因此，该研究建议肥料在22N：22P：30K：5S kg / ha和33n：33p：33p：45k：7s kg / ha用b.培养婴儿菠菜时被考虑枯草芽孢杆菌接种。
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