Premise:The family Salicaceae has proved taxonomically challenging, especially in the genus Salix, which is speciose and features frequent hybridization and polyploidy. Past efforts to reconstruct the phylogeny with molecular barcodes have failed to resolve the species relationships of many sections of the genus.Methods:We used the wealth of sequence data in the family to design sequence capture probes to target regions of 300-1200 bp of exonic regions of 972 genes.Results:We recovered sequence data for nearly all of the targeted genes in three species of Populus and three species of Salix. We present a species tree, discuss concordance among gene trees, and present population genomic summary statistics for these loci.Conclusions:Our sequence capture array has extremely high capture efficiency within the genera Populus and Salix, resulting in abundant phylogenetic information. Additionally, these loci show promise for population genomic studies.? 2020 Sanderson et al. Applications in Plant Sciences published by Wiley Periodicals LLC on behalf of Botanical Society of America.
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机译：前提：Salicaceae的家族已经证明了分类，特别是在Salix属中，其是常见的杂交和多倍体的特征。过去重建与分子条形码的系统发生的努力未能解决这些属的许多部分的物种关系。我们使用了家庭中的大量序列数据来设计序列捕获探头，以瞄准偏振的目标区域为300-1200 bp 972个基因的区域。结果：我们在三种杨树和三种盐渍中恢复几乎所有靶向基因的序列数据。我们展示了一种物种树，讨论基因树的一致性，并呈现这些基因座的群体基因组概要统计数据。结论：我们的序列捕获阵列具有极高的捕获效率，在Genera Populus和Salix内具有极高的捕获效率，导致了丰富的系统发育信息。此外，这些基因座表明人口基因组研究的承诺。 2020 Sanderson等。 Wiley期刊LLC代表美国植物学会出版的植物科学申请。
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