In Brazil, Rhipicephalus sanguineus resistance to some pyrethroids have been detected, motivating research on new phyto-insecticides such as essential oil from Tetradenia riparia leaves (EOL) and flower buds (EOFB).The essential oils were obtained by hydrodistillation (3h) and identified by GC/MS.In addition, a multivariate exploratory analysis was done to determine the analysis of the major compounds (PCA).The bioassays on R.sanguineus larvae were done by immersion test at different EO concentrations which ranged from 50,000 to 0.47 mg/mL (v/v).The action mechanism of EOs were determined by bioautographic method evaluating the inhibitory potential on the acetylcholinesterase enzyme.The EO yield in leaves was 0.29±0.22 (%) and in flower buds 0.38±0.17 (%).The class projections showed oxygenated sesquiterpenes (43.62%) and diterpenes (15.60%) in EOFB, and hydrocarbon sesquiterpenes (26.44%) and oxygenated monoterpenes (16.44%) in EOL.Four components presented a greater distancing of mass flow: fenchone (11.57 and 6.01 %), α-cadinol (12.21 and 13.69 %), 14-hidroxy-9-epi-caryophyllene (8.56 and 15.38 %), and caryophyllene oxide (1.32 and 4.50 %) in EOL and EOFB, respectively.The lethal concentrations (LCs) to kill R.sanguineus larvae were (LC50: 2.18±0.24 and LC99.9: 9.98±0.10 mg/mL) for EOL, and (LC50: 5.36±2.50 and LC99.9: 20.12±0.54 mg/mL) for EOFB.The action mechanism of EOs by bioautographic methods indicated an inhibition of 0.70 mg/mL (EOL) and 1.40 mg/mL (EOFB) on the acetylcholinesterase enzyme (AChE).Therefore, this species can be considered promising to be part of the chemical larvicides to control this ectoparasite.
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