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An Electrochemical Benzylpenicillin Biosensor Based on β- Lactamase and Fullerene Supported by A Bilayer Lipid Membrane

机译:基于双层脂质膜负载的β-内酰胺酶和富勒烯的电化学苄基培素生物传感器

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A novel biosensor with high sensitivity for the electrochemical detection of benzylpenicillin wassuccessfully constructed by immobilizing β-Lactamase on a fullerene (C60) supported bilayer lipidmembrane (C60-s-BLM) modified gold electrode (GE). β-Lactamase was well immobilized on theelectrode surface because of the good biocompatibility of the BLM. C60 was used as an electron transferenhancer and an efficient immobilization matrix for the sensitivity enhancement. Transmission electronmicroscope (TEM) and cyclic voltammetry (CV) were used to characterize the surface morphology andthe catalytic performance of the electrode, respectively. The results showed that the sensor had a goodlinearity in the range of 1.9-223.3 ng/L and a detection limit of 0.6 ng/L..
机译:一种新的生物传感器,具有高灵敏度,具有高灵敏度,用于通过固定β-内酰胺基于富勒烯(C60)的双层脂质膜(C60-S-BLM)修饰的金电极(GE)固定β-内酰胺酶的电化学检测高灵敏度。由于BLM的良好生物相容性,β-内酰胺酶在电极表面上良好固定在电极表面上。 C60用作电子转移素和敏感性增强的有效固定基质。透射电子显微镜(TEM)和循环伏安法(CV)分别用于表征电极的表面形态和催化性能。结果表明,传感器的良好程度在1.9-223.3ng / L的范围内,检测限为0.6 ng / l ..

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