Carbon-based nanomaterials possess a remarkably high potential for biomedical applications due to their physical properties; however, their detrimental effects on reproduction are also concerned. Several reports indicate the toxicity of carbon nanotubes (CNT); nevertheless, their impact on intracellular organelles in the male reproductive organs has not been fully elucidated. Herein, we report on the reprotoxicity of single-walled (SWCNT) and multi-walled carbon nanotubes (MWCN) on several intracellular events and histological criteria in pubertal male BALB/c mice orally treated with 0, 10, and 50 mg/kg/day doses for five weeks. Biomarkers of oxidative stress and mitochondrial functionality, histopathological alterations, and epididymal sperm characteristics were determined. Oral administration of CNTs at 10, and 50 mg/kg evoked a significant decrement in weight coefficient, sperm viability and motility, hypo-osmotic swelling (HOS) test, sperm count, mitochondrial dehydrogenase activity, ATP content, total antioxidant capacity, and GSH/GSSH ratio in the testis and epididymal spermatozoa. On the other hand, percent abnormal sperm, testicular and sperm TBARS contents, protein carbonylation, ROS formation, oxidized glutathione level, and sperm mitochondrial depolarization were considerably increased. Significant histopathological and stereological alterations in the testis occurred in the groups challenged with CNTs. The current findings indicated that oxidative stress and mitochondrial impairment might substantially impact CNTs-induced reproductive system injury and sperm toxicity. The results can also be used to establish environmental standards for CNT consumption by mammals, produce new chemicals for controlling the rodent populations, and develop therapeutic approaches against CNTs-associated reproductive anomalies in the males exposed daily to these nanoparticles.
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机译：由于其物理性质，碳基纳米材料具有显着的生物医学应用的潜力;然而，它们对繁殖的不利影响也是关注的。几份报告表明碳纳米管（CNT）的毒性;然而，它们对雄性生殖器官中对细胞内细胞器的影响尚未完全阐明。在此，我们报告单壁（SWCNT）和多壁碳纳米管（MWCN）对几种细胞内事件和组织学标准的普浴阳性BALB / C小鼠的组织学标准的报告报告，用0,10,10mg / kg /日剂量五周。确定了氧化应激和线粒体功能，组织病理学改变和附睾精子特征的生物标志物。口服施用10，50mg / kg的CNT诱发重量系数，精子活力和运动，低渗肿胀（HOS）试验，精子计数，线粒体脱氢酶活性，ATP含量，总抗氧化能力和GSH的显着降低/ GSSH比率在睾丸和附睾精子中。另一方面，异常精子百分比，睾丸和精子TBAR含量，蛋白质羰基化，ROS形成，氧化谷胱甘肽水平和精子线粒体去极化得到显着增加。睾丸中的显着组织病理学和立体或立体学改变发生在攻击中CNTs攻击的组中。目前发现表明氧化应激和线粒体损伤可能会显着影响CNTs诱导的生殖体系损伤和精子毒性。结果还可用于建立哺乳动物CNT消费的环境标准，产生用于控制啮齿动物群体的新化学品，并在每天暴露于这些纳米颗粒的雄性中，开发针对CNTS相关的生殖异常的治疗方法。
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