Brain function depends on patterns of synaptic input and neurocircuits,which are accompanied by transient changes in free intracellular calcium concentration (Resendez et al.,2015).A recent topic of interest has been the illumination of neuronal circuit activity by calcium imaging in vivo.As a new biological tool for recording behavioral signals in the animal brain,in vivo calcium imaging is performed to monitor neuronal activity based on intracellular calcium.In vivo calcium imaging is an advanced technique by which individual cells and/or even subcellular compartments can be observed in real time.Thus,it readily enables simultaneous recording of individual cells and subcellular compartments.
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