BACKGROUND:Bisphosphonates (BPs) are widely used as anti-bone-resorptive agents. Despite the great benefits of BPs, they may cause local and systemic adverse side effects.OBJECTIVES:The aim of this study was to evaluate the histopathological effect zoledronic acid (ZA), which belongs to BPs, has on the intrinsic tongue muscles in a rat model.MATERIAL AND METHODS:A total of 30 adult male albino rats were divided into 3 groups (10 rats each): group I served as a control; group II was given an intraperitoneal (i.p.) injection of 0.2 mg/kg of ZA once per week for 3 weeks; and group III received the same dosage of ZA, but for 8 weeks. After the animals were euthanized, the tongue tissue was dissected and examined histologically, histochemically and immunohistochemically.RESULTS:Histologically, a normal architecture of the muscle fascicles was observed in the control group. Group II showed degenerated muscle fibers with an indistinct sarcolemma. In group III, the muscle fibers were degenerated with severe sarcoplasmic dissolution. The histochemical examination using Masson's trichrome (MT) demonstrated a significant increase in collagen fibers in groups II and III as compared to the control group. The immunohistochemical results revealed a statistically significantly higher expression of nuclear factor kappa B (NF?κB) in the ZA-treated groups (II and III) as compared to the control group, with the highest mean value recorded in group III.CONCLUSIONS:Zoledronic acid induced histopathological changes to the intrinsic tongue muscles, and this effect was exaggerated with a longer duration of administration.
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机译：背景：双膦酸盐（BPS）被广泛用作抗骨吸收剂。尽管BPS的好处，但它们可能导致局部和全身不良副作用。目的：本研究的目的是评估属于BPS的组织病理学酸（ZA），对大鼠的内在舌肌呈上舌肌模型。材料和方法：将30名成年雄性白化大鼠分为3组（每只大鼠10只）：I基团用作控制;第II族被拘留为每周0.2mg / kg Za的腹膜内（I.P.）。和第三组接受相同的Za剂量，但8周。在使动物进行安乐死之后，对舌组织进行解剖和检查组织化学和免疫组织化学。结果：组织学上，在对照组中观察到肌肉束的正常结构。第II族显示肌肉纤维的退化肌纤维，含糊不清的SARCOMMA。在III组中，肌肉纤维具有严重的肌肉溶解来退化。使用Masson的richrome（MT）的组织化学检查表明，与对照组相比，II和III组中的胶原纤维显着增加。免疫组织化学结果表明，与对照组相比，核因子Kappa（III）中的核因子Kappa B（NFακB）表达核因子Kappa B（NFβb）表达，III组中记录的最高平均值。结论：Zoledronic酸诱导对本征舌肌的组织病理学变化，并且这种效果夸大了较长的给药持续时间。
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