Biological and microbiological activities of isolated <ce:italic>Enterobacter</ce:italic> sp. ACD2 exopolysaccharides from Tabuk region of Saudi Arabia

Mikhlid H. Almutairi; Mohamed M.I. Helal

摘要

Several forms of bacterial exopolysaccharides (EPSs) have been reported as having industrial and medical benefits. Previously, our group had achieved a high yield of EPS production (8.6?gm/L) fromEnterobactersp. isolated from the marine environment of Haqel Beach, in the Tabuk region of Saudi Arabia, by optimizing its culture conditions. Then, the strain selected was identified using conventional methods and confirmed by molecular characterization that used 16S Ribosomal RNA (16S rRNA) gene sequencing. In the present study, we determined the composition of this EPS and evaluated its anticoagulation, fibrinolytic, antimicrobial, and prebiotic activities. We determined the monosaccharide moieties of this EPS using acid hydrolysis by qualitative and quantitative paper chromatography (PC). The monosaccharide composition of this EPS was almost 25% glucose, 25% galactose, 40% fucose, in addition to 10% uronic acid, with traces of fructose. Sulfation modification was applied to the EPS produced. The biological and microbiological (anticoagulation, fibrinolytic, antimicrobial, and prebiotic) activities of both the native and sulfated EPS were investigated and both exhibited higher antibacterial activity againstStaphyllococcus aureusandEscherichia coli, although neither form displayed antifungal activity againstCandida albicans. The minimum inhibitory concentration (MIC) of the native and sulfated EPS that sustained the highest bacterial inhibition zone was 15?mg/dl. No prebiotic activities were recorded for either EPS. In conclusion, the biopolymer produced has significant biological activities that require further adaptation for practical and industrial applications.

机译：据报道，几种形式的细菌外碳（EPS）具有工业和医疗益处。此前，我们的小组已达到高产EPS生产（8.6〜GM / L）的EPSOB形。从Haqel海滩的海洋环境中孤立，在沙特阿拉伯的塔克地区，通过优化其文化条件。然后，使用常规方法鉴定所选择的菌株并通过使用16S核糖体RNA（16SRRNA）基因测序的分子表征来证实。在本研究中，我们确定了该EPS的组成，并评估了其抗凝，纤维蛋白溶解，抗微生物和益生元活性。我们使用定性和定量纸张色谱（PC）使用酸水解来确定该EPS的单糖部分。该EP的单糖组合物几乎是25％葡萄糖，25％半乳糖，40％岩藻糖，除了10％核酸外，具有果糖痕量。将硫化修饰应用于所产生的EPS。研究了天然和硫酸钠EPS的生物和微生物（抗凝血，纤维蛋白溶解，抗菌剂和益生元）活性，并且均表现出较高的抗菌活性，抵御抗菌活性AUSTUSANDESCHERICA COLI，尽管既没有表现出抗真菌活性的抗真菌活性。持续最高细菌抑制区的天然和硫酸盐EPS的最小抑制浓度（MIC）为15〜Mg / dL。任何eps都没有记录益生元活动。总之，生物聚合物产生的具有显着的生物活性，需要进一步适应实际和工业应用。

Biological and microbiological activities of isolated Enterobacter sp. ACD2 exopolysaccharides from Tabuk region of Saudi Arabia

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