Chemical composition, antioxidant, anti-inflammatory and cytotoxic effects of Chondrus crispus species of red algae collected from the Red Sea along the shores of Jeddah city

Maha I. Alkhalaf

摘要

The Red Sea coast is rich in different types of seaweeds, which are suggested to possess medicinal properties. In this study, methanolic extract of Chondrus crispus (MEC), a species of red algae collected from the southeast coast of Jeddah, Saudi Arabia was prepared and analysed for its chemical composition, antioxidant, anti-inflammatory and cytotoxic properties. HPLC analysis revealed that flavonoids were found to be predominant constituents of MEC followed by phenols and tannins. Among phenols, catechin, gallic andp-coumaric acids were found to be major components of MEC. The MEC at 200?μg/mL concentration significantly decreased the levels of free radicals including 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and displayed significant total antioxidant capacity (TAC). The MEC also significantly inhibited the denaturation of bovine serum albumin (BSA), indicating its anti-inflammatory potential. Furthermore, hepatocellular carcinoma (HepG2) and adenocarcinoma human alveolar (A549) cells treated with MEC exhibited 81.9% and 71.8% cytotoxic cell death respectively as compared to 69% cell death found in cells treated with reference drug sorafinib. Collectively, the data revealed that flavonoids, phenols and tannins are the major chemical constituents of chondrus crispus species of red algae. Further, the methanolic extract of this species was found to exhibit significant antioxidant, anti-inflammatory and cytotoxic properties in several human cancer cell lines. Thus, red algae commonly found along the shore of Red Sea can be utilized for its medicinal value.

机译：红海海岸富含不同类型的海藻，建议具有药用特性。在这项研究中，制备并分析了沙特阿拉伯东南海岸收集的软骨菌脆节（MEC）的甲醇提取物，并分析了其化学成分，抗氧化，抗炎和细胞毒性。 HPLC分析显示，发现黄酮类化合物是MEC的主要成分，然后是酚和单宁。在酚类中，发现儿茶素，神经生长和香豆素酸被发现是MEC的主要组分。 200μg/ ml浓度的MEC显着降低了自由基的水平，包括2,2-二苯基-1-Picryl-肼 - 水合物（DPPH）和2,2'-氮杂-HIS（3-乙基苯并噻唑啉-6-磺酸酸（ABTS），并显示出显着的总抗氧化能力（TAC）。MEC也显着抑制了牛血清白蛋白（BSA）的变性，表明其抗炎潜力。此外，肝细胞癌（Hepg2）和腺癌人肺泡（A549用MEC处理的细胞分别表现出81.9％和71.8％的细胞毒性细胞死亡，而在用参考药物索拉阿法尼布治疗的细胞中发现的细胞中发现的69％死亡。集体显示，黄酮类化合物，酚类和单宁是软骨菌脆节的主要化学成分红藻类种类。此外，发现该物种的甲醇提取物在几种人类癌细胞系中表现出显着的抗氧化剂，抗炎和细胞毒性特性。因此，红藻通常是沿着红海的岸边可用于其药用价值。

Chemical composition, antioxidant, anti-inflammatory and cytotoxic effects of Chondrus crispus species of red algae collected from the Red Sea along the shores of Jeddah city

摘要

著录项

相关主题

期刊订阅