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>Mining of halo-tolerant plant growth promoting rhizobacteria and their impact on wheat ( Triticum aestivum L.) under saline conditions
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Mining of halo-tolerant plant growth promoting rhizobacteria and their impact on wheat ( Triticum aestivum L.) under saline conditions
Plant growth promoting rhizobacteria have the potential to mitigate abiotic stressors to improve plant productivity. The current study was conducted to isolate and characterize halo-tolerant PGPR strains from the native plants of Khewra salt mine in Pakistan. Two halo-tolerant bacterial strains (SBN01 and SBN02) were purified, examinedin vitrofor their plant growth-promoting traits, and inoculated on wheat plants to evaluate their beneficial effects. Both isolates were able to produce indole-acetic acid in tryptophan-supplemented media, both were N2fixers and capable of solubilizing phosphate. These isolates also showed positive results for catalase, gelatinase, and ammonia, except for protease activity. Based on16S rRNAgene sequence analysis, SBN01 and SBN02 isolates showed 100% homology withAlcaligenes faecalis. Inoculation studies on wheat plant showed that these isolates maintained ionic imbalance by regulating Na+and K+ions and significantly increased growth parameters and plant biomass by decreasing reactive oxygen species induced lipid peroxidation while increased accumulation of osmolyte, photosynthetic pigments and improved photosystem II efficiency as compared to uninoculated plants. These results showed that isolated halo-tolerant rhizobacterial strains could improve plant growth and enhance tolerance during a stressful environment. Thus, they can be used as potential cost-effective candidates for growing wheat in salt-affected areas.
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机译：植物生长促进无根杆菌有可能减轻非生物压力源以改善植物生产率。目前的研究是在巴基斯坦在Khewra Salt Mine的天然植物中分离和表征卤素耐受性PGPR菌株。纯化两种卤素细菌菌株（SBNO和SBNO 2），将其植物生长促进的特征纯化，调查培养物，并接种在小麦植物上以评估它们的有益效果。两种分离物能够在色氨酸补充剂中生产吲哚 - 乙酸，两者都是N 2掺杂物，并且能够溶解磷酸盐。除蛋白酶活性外，这些分离物还显示出过氧化氢酶，明胶酶和氨的阳性结果。基于16S的Rrnagene序列分析，SBN01和SBN02分离物显示出100％的同源性valcaligenes粪便。对小麦工厂的接种研究表明，这些分离物通过调节Na +和K +离子来保持离子不平衡，通过降低反应性氧物种诱导的脂质过氧化，同时增加渗透性，光合颜料和改善的照相II效率，显着增加了生长参数和植物生物质。与未征收的植物相比。这些结果表明，分离的卤代耐卤代菌菌株可以改善植物生长和增强压力环境中的耐受性。因此，它们可用作含盐影响地区种植小麦的潜在成本效益的候选者。
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