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首页> 外文期刊>Emerging Infectious Diseases >CrAssphage as a Novel Tool to Detect Human Fecal Contamination on Environmental Surfaces and Hands
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CrAssphage as a Novel Tool to Detect Human Fecal Contamination on Environmental Surfaces and Hands

机译：韵文作为一种新颖的工具，用于检测环境表面和手的人类粪便污染

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CrAssphage is a recently discovered human gut-associated bacteriophage. To validate the potential use of crAssphage for detecting human fecal contamination on environmental surfaces and hands, we tested stool samples (n = 60), hand samples (n = 30), and environmental swab samples (n = 201) from 17 norovirus outbreaks for crAssphage by real-time PCR. In addition, we tested stool samples from healthy persons (n = 173), respiratory samples (n = 113), and animal fecal specimens (n = 68) and further sequenced positive samples. Overall, we detected crAssphage in 71.4% of outbreak stool samples, 48%-68.5% of stool samples from healthy persons, 56.2% of environmental swabs, and 60% of hand rinse samples, but not in human respiratory samples or animal fecal samples. CrAssphage sequences could be grouped into 2 major genetic clusters. Our data suggest that crAssphage could be used to detect human fecal contamination on environmental surfaces and hands.
机译：韵律是最近发现的人体肠道相关的噬菌体。为了验证在环境表面和手上检测人类粪便污染的潜在用途，我们测试了大便样品（n = 60），手样（n = 30），和环境拭子样本（n = 201），从17毫诺病毒爆发通过实时PCR的韵律。此外，我们测试了从健康人（n = 173），呼吸样品（n = 113）和动物粪便样本（n = 68）的粪便样本并进一步测序阳性样品。总体而言，我们在71.4％的爆发粪便样品中检测到韵文，48％-68.5％的粪便样本来自健康人，48.2％的环境拭子，60％的手漂洗样品，但不在人类呼吸样品或动物粪便样本中。韵律序列可以分为2个主要遗传簇。我们的数据表明，奇格文格可用于检测环境表面和手的人类粪便污染。

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