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首页> 外文期刊>Journal of bacteriology >Identification of a Transcriptomic Network Underlying the Wrinkly and Smooth Phenotypes of Vibrio fischeri
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Identification of a Transcriptomic Network Underlying the Wrinkly and Smooth Phenotypes of Vibrio fischeri

机译：鉴定覆盖皱纹和平滑表型的转发组网络

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Vibrio fischeri is a cosmopolitan marine bacterium that oftentimes displays different colony morphologies, switching from a smooth to a wrinkly phenotype in order to adapt to changes in the environment. This wrinkly phenotype has also been associated with increased biofilm formation, an essential characteristic for V. fischeri to adhere to substrates, to suspended debris, and within the light organs of sepiolid squids. Elevated levels of biofilm formation are correlated with increased microbial survival of exposure to environmental stressors and the ability to expand niche breadth. Since V. fischeri has a biphasic life history strategy between its free-living and symbiotic states, we were interested in whether the wrinkly morphotype demonstrated differences in its expression profile in comparison to the naturally occurring and more common smooth variant. We show that genes involved in major biochemical cascades, including those involved in protein sorting, oxidative stress, and membrane transport, play a role in the wrinkly phenotype. Interestingly, only a few unique genes are specifically involved in macromolecule biosynthesis in the wrinkly phenotype, which underlies the importance of other pathways utilized for adaptation under the conditions in which Vibrio bacteria are producing this change in phenotype. These results provide the first comprehensive analysis of the complex form of genetic activation that underlies the diversity in morphologies of V. fischeri when switching between two different colony morphotypes, each representing a unique biofilm ecotype. IMPORTANCE The wrinkly bacterial colony phenotype has been associated with increased squid host colonization in V. fischeri. The significance of our research is in identifying the genetic mechanisms that are responsible for heightened biofilm formation in V. fischeri. This report also advances our understanding of gene regulation in V. fischeri and brings to the forefront a number of previously overlooked genetic networks. Several loci that were identified in this study were not previously known to be associated with biofilm formation in V. fischeri.
机译：Vibrio Fischeri是一个过度的海洋细菌，通常会显示不同的菌落形态，从平滑到碎片表型切换，以适应环境的变化。这种碎片表型也与生物膜形成的增加有关，V.Fischeri的基本特征是粘附在底物上，悬浮的碎片，以及在Sepiolid鱿鱼的光器官内。生物膜形成的升高与环境压力源的微生物存活增加以及扩大利基广度的能力相关。由于V.Fischeri在自由生活和共生状态之间具有双相生活历史策略，我们对其表达概况的奇异和更常见的光滑变体相比，我们对其表达概况的差异感兴趣。我们展示了参与主要生化级联的基因，包括参与蛋白质分选，氧化应激和膜运输的基因，在皱纹表型中发挥作用。有趣的是，只有少数独特的基因特异性地参与碎片表型中的大分子生物合成，这使得在弧菌细菌在表型产生这种变化的条件下利用了适应的其他途径的重要性。这些结果提供了对复杂遗传活化形式的综合分析，该遗传活化的复杂性激活是在两种不同殖民地Morothy型之间切换时的V.Fischeri的形态的多样性。每个代表独特的生物膜生态型。重要性皱纹细菌菌落表型已与V.Fischeri的鱿鱼宿主定植增加有关。我们的研究的重要性是识别负责在V.Fischeri中的增强生物膜形成的遗传机制。本报告还提出了我们对V.Fischeri的基因调节的理解，并为最重要的遗传网络带来了最前沿。在本研究中鉴定的几个基因座以前未熟悉与V.Fischeri的生物膜形成相关。

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