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首页> 外文期刊>International Journal of Forestry Research >cpDNA-Gene-Sequence-Based Genetic Diversity, Population Structure, and Gene Flow Analysis of Ethiopian Lowland Bamboo (Bambusinea: Oxytenanthera abyssinica (A. Rich.) Munro)
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cpDNA-Gene-Sequence-Based Genetic Diversity, Population Structure, and Gene Flow Analysis of Ethiopian Lowland Bamboo (Bambusinea: Oxytenanthera abyssinica (A. Rich.) Munro)

机译：基于CpDNA-基因序列的遗传多样性，人口结构和基因流分析埃塞俄比亚低地竹（Bambusinea：Oxytenanthera Abyssinica（A.富人）Munro）

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摘要

Background . As a member of Poaceae and subfamily Bambusoideae, Ethiopian lowland bamboo ( Oxytenanthera abyssinica ) is one of the most important nontimber forest resources or a potential alternative to wood and wood products. Ethiopia contributes 86% of the total area of bamboo on the continent, Africa, and 7% of the world. O. abyssinica in Ethiopia accounts for 85% of the total national coverage of bamboo. Several studies have been performed on the genetic diversity and population structure analysis of various bamboo species throughout the world but almost nothing in Ethiopia and O. abyssinica . Methods . Young fresh leaves of O. abyssinica from thirteen natural lowland bamboo growing areas across the country were collected. DNA was isolated using a modified CTAB DNA isolation method. Three cpDNA gene sequences (matK, ndhF3, and rps16) were used for the study. PCR products were analyzed, purified, and pair-end sequenced to calculate AC/GC content, average number of nucleotide differences ( k ), nucleotide diversity ( π ) and population mutation rates per 100 sites ( ), InDel (Insertion-Deletion), DNA divergence, gene flow, and genetic differentiation. Results . Metekel Zone was found to have extremely higher k , π , and . Higher frequency of genetic differentiation was found between Metekel Zone vs. the distant populations. Higher frequency of gene flow was found between Assosa Zone vs. Oromia populations. Kurmuk haplotype from gaps or missing data considered and Bambasi haplotype from not considered has descendants around them. Conclusion . Using sequences of cpDNA genes, populations of O. abyssinica collected in Ethiopia show clear diversity based on their geographic location. Metekel Zone was found to have the most diverse population, Assosa Zone has been found to be the source of evolution of O. abyssinica , and Gambella population shows a difference from other O. abyssinica populations.
机译：背景 。作为Poaceae和Subfamily Bambusoideae的成员，埃塞俄比亚低地竹（Oxyttenanthera Abyssinica）是最重要的未森林资源或木材和木材产品的潜在替代品之一。埃塞俄比亚在非洲大陆，非洲和世界7％的竹子占竹子总面积的86％。 O.埃塞俄比亚的Abyssinica占全国竹子覆盖率的85％。对全世界各种竹种的遗传多样性和人口结构分析进行了几项研究，但几乎在埃塞俄比亚和O. Abyssinica中几乎没有。方法 。从全国的十三自然低地竹生长地区的o. abysinica的年轻新鲜的叶子被收集。使用改性的CTAB DNA分离方法分离DNA。使用三种CPDNA基因序列（MATK，NDHF3和RPS16）进行研究。分析PCR产物，纯化和对末端测序以计算AC / GC含量，平均核苷酸差（K），核苷酸多样性（π）和每100位点（），indel（插入缺失）的群体突变率， DNA分歧，基因流动和遗传分化。结果 。发现Metekel区具有极高的K，π和。在Metekel区与远处群体之间发现了越遗传分化的较高频率。在Assosa区与Oromia群体之间发现了较高的基因流频率。来自差距的Kurmuk单倍型或缺失的数据和来自未考虑的Bambasi单倍型具有周围的后代。结论 。使用CPDNA基因的序列，埃塞俄比亚收集的O. Abysinica的种群显示出基于其地理位置的明显多样性。发现迈克尔区拥有最多的人口，Assosa区已被发现是O. Abysinica的演变来源，而Gambella人口显示与其他O. Abyssinica群体的差异。

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