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首页> 外文期刊>Journal of oncology >CEP55 Positively Affects Tumorigenesis of Esophageal Squamous Cell Carcinoma and Is Correlated with Poor Prognosis
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CEP55 Positively Affects Tumorigenesis of Esophageal Squamous Cell Carcinoma and Is Correlated with Poor Prognosis

机译：Cep55积极影响食管鳞状细胞癌的肿瘤内核，并与预后差相关

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Centrosomal protein 55 (CEP55) is a centrosome- and midbody-associated protein that is overexpressed in several cancers. However, the underlying molecular mechanism of CEP55-mediated progression and metastasis of esophageal squamous cell carcinoma (ESCC) is not clear. In the current study, we detected CEP55 mRNA by qRT-PCR while protein expression was detected by western blot analysis and immunohistochemistry (IHC). In addition, we knocked down CEP55 and investigated the ability of CEP55 to affect colony formation and migration. Here, we report that CEP55 mRNA and protein expression was significantly increased in ESCC. IHC staining showed that CEP55 expression correlated with TNM stage ( ) and lymph node metastases ( ). According to overall survival (OS) and disease-free survival (DFS), patients whose tumors expressed a higher level of CEP55 had a poorer prognosis than those with low expression level of CEP55. A multivariate analysis revealed that CEP55 expression was an independent prognostic indicator for patients with ESCC. Knockdown of CEP55 decreased the colony formation ability and migration of ESCC cells and also reduced the phosphorylation of Src, FAK, and ERK. Therefore, our study implied that CEP55 may be a valuable biomarker and a potential target in the treatment of patients with ESCC.
机译：Centrosomal蛋白55（Cep55）是一种在几种癌症中过表达的中心和中间体相关蛋白。然而，Cep55介导的进展和食管鳞状细胞癌（ESCC）转移的潜在分子机制尚不清楚。在目前的研究中，我们通过QRT-PCR检测Cep55 mRNA，而蛋白质表达被Western印迹分析和免疫组织化学（IHC）检测到。此外，我们击倒了CEP55并调查了CEP55影响殖民地形成和迁移的能力。在这里，我们报告说，EP55 mRNA和蛋白质表达在ESCC中显着增加。 IHC染色表明CEP55表达与TNM阶段（）和淋巴结转移（）相关。根据整体生存（OS）和无病生存（DFS），肿瘤表达较高水平的患者的CEP55的预后比CEP55表达水平的预后较差。多变量分析表明，CEP55表达是ESCC患者的独立预后指标。 CEP55的敲低下降降低了ESCC细胞的菌落形成能力和迁移，并降低了SRC，FAK和ERK的磷酸化。因此，我们的研究暗示CEP55可以是有价值的生物标志物和治疗ESCC患者的潜在目标。

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