...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Brucella abortus as a potential vaccine candidate: induction of interleukin-12 secretion and enhanced B7.1 and B7.2 and intercellular adhesion molecule 1 surface expression in elutriated human monocytes stimulated by heat-inactivated B. abortus.
【24h】

Brucella abortus as a potential vaccine candidate: induction of interleukin-12 secretion and enhanced B7.1 and B7.2 and intercellular adhesion molecule 1 surface expression in elutriated human monocytes stimulated by heat-inactivated B. abortus.

机译：Brucella abortus作为潜在的疫苗候选者：诱导白细胞介素-12分泌和增强的B7.1和B7.2和B7.2的细胞间粘附分子1在由热灭活的B. abortive刺激的产物刺激的产物单核细胞中的细胞间表达。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Development of a vaccine which is capable of generating a strong cellular immune response associated with gamma interferon (IFN-gamma) production and cytotoxic T-cell development requires that the immunogen be capable of inducing the secretion of interleukin-12 (IL-12), which is a pivotal factor for the differentiation of Th1 or Tc1 cells. We have previously shown that the heat-inactivated gram-negative bacterium Brucella abortus can induce IFN-gamma secretion by T cells. In the present study, we demonstrate that B. abortus and lipopolysaccharide (LPS) from B. abortus can induce IL-12 p40 mRNA expression and protein secretion by human elutriated monocytes (99% pure). p40 mRNA was detected within 4 h, and p40 protein could be measured at 24 h. This induction was abrogated by anti-CD14 monoclonal antibody, suggesting that monocytes recognize B. abortus via their receptor for LPS. The biological activity of IL-12 secreted by B. abortus-stimulated monocytes was demonstrated by its ability to upregulate IFN-gamma mRNA expression in T cells separated from monocytes and B. abortus by a transwell membrane. The B. abortus-induced IL-12 also enhanced NK cytolytic activity against K562 target cells. B. abortus was shown to rapidly increase the expression of the costimulatory molecules B7.1 and B7.2 and intercellular adhesion molecule 1 on human monocytes. Together, these data indicate that B. abortus can directly activate human monocytes and provide the cytokine milieu which would direct the immune response towards Th1-Tc1 differentiation.
机译：的疫苗，其能够生成与γ干扰素（IFN-γ）的产生和细胞毒性T细胞发育相关的强烈的细胞免疫应答的发展需要免疫原能够诱导白介素-12（IL-12）的分泌的，这是Th1型或Tc1的细胞分化的关键因素。我们先前已经表明，热灭活的革兰氏阴性细菌流产布鲁氏菌可以通过T细胞诱导IFN-γ分泌。在本研究中，我们证明B.流产和从B.流产布鲁氏菌脂多糖（LPS）可诱导IL-12 p40的mRNA表达和蛋白质分泌由人单核细胞淘洗（99％纯）。 p40的mRNA的4小时内检测到的，和p40蛋白可以在24小时进行测量。这个诱导通过抗CD14单克隆抗体废除，表明单核细胞通过它们的受体对LPS识别B.流产。 IL-12的由B.分泌的生物活性流产布鲁氏菌，单核细胞刺激物通过它能够在T细胞中上调的IFN-γmRNA表达表明从单核细胞和B.分离流产布鲁氏菌通过跨孔膜。的流产布鲁氏菌B.诱导的IL-12也增强NK细胞裂解活性对K562靶细胞。 B.流产布鲁氏菌显示出迅速增加共刺激分子B7.1和B7.2和ICAM 1上人单核细胞中的表达。总之，这些数据表明，流产布鲁氏菌B.能直接激活人类单核细胞，并提供细胞因子环境这将引向的Th1-Tc1的分化的免疫应答。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号