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首页> 外文期刊>Infection and immunity >Mannan-Specific Immunoglobulin G Antibodies in Normal Human Serum Accelerate Binding of C3 to Candida albicans via the Alternative Complement Pathway
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Mannan-Specific Immunoglobulin G Antibodies in Normal Human Serum Accelerate Binding of C3 to Candida albicans via the Alternative Complement Pathway

机译：曼南特异性免疫球蛋白G正常人血清中的抗体通过替代补体途径加速C3与念珠菌醛糖苷的结合

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Candida albicans activates the classical and alternative complement pathways, leading to deposition of opsonic complement fragments on the cell surface. Our previous studies found that antimannan immunoglobulin G (IgG) in normal human serum (NHS) allows C. albicans to initiate the classical pathway. The purpose of this study was to determine whether antimannan IgG also plays a role in initiation of the alternative pathway. Pooled NHS was rendered free of classical pathway activity by chelation of serum Ca2+ with EGTA alone or in combination with immunoaffinity removal of antimannan antibodies. Kinetic analysis revealed a 6-min lag in detection of C3 binding to C. albicans incubated in EGTA-chelated NHS, compared to a 12-min lag in NHS that was both EGTA chelated and mannan absorbed. The 12-min lag was shortened to 6 min by addition of affinity-purified antimannan IgG. The accelerating effect of antimannan IgG on alternative pathway initiation was dose dependent and was reproduced in a complement binding reaction consisting of six purified proteins of the alternative pathway. Both Fab and F(ab′)2 fragments of antimannan IgG facilitated alternative pathway initiation in a manner similar to that observed with intact antibody. Immunofluorescence analysis showed that addition of antimannan IgG to EGTA-chelated and mannan-absorbed serum promoted an early deposition of C3 molecules on the yeast cells but had little or no effect on distribution of the cellular sites for C3 activation. Thus, antimannan IgG antibodies play an important regulatory role in interactions between the host complement system and C. albicans.
机译：念珠菌蛋白（Candida albicans 激活经典和替代的补体途径，导致Opsonic补体片段沉积在细胞表面上。我们以前的研究发现，正常人血清（NHS）中的抗血管免疫球蛋白G（IgG）允许 C. albicans 启动经典途径。本研究的目的是确定Antimannan IgG是否在替代途径的启动中也发挥作用。通过单独的血清Ca 2 + 螯合血清Ca 2 + / sup>或与免疫亲和性除去抗血管抗体的免疫亲和性，通过培养型途径活性提供典型的NH。动力学分析显示，在检测到 C的C3结合时揭示了6分钟的滞后。与NHS中的12分钟的滞后相比，在EGTA螯合NHS中孵育在EGTA螯合和甘露甘露甘油中的12分钟内。通过添加亲和力纯化的抗血管IgG，将12分钟的滞后缩短至6分钟。 Antimannan IgG对替代途径起始的加速效果是依赖性剂量，并在由六种替代途径的六种纯化的蛋白质组成的补体结合反应中进行。 Fab和f（ab'） 2 antimannan IgG的碎片促进替代途径引发，其方式类似于用完整抗体观察到的方式。免疫荧光分析表明，向EGTA螯合和甘露甘露甘露植物吸收的血清添加抗血管烷IgG促进了C3分子在酵母细胞上的早期沉积，但对C3活化的细胞位点分布几乎没有或没有影响。因此，Antimannan IgG抗体在宿主补体系统和 C之间的相互作用中起重要的调节作用。 albicans。

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