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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Role of Immunohistochemical Markers p53 and Ki-67 in Grading of Glial Tumours: A Prospective Study
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Role of Immunohistochemical Markers p53 and Ki-67 in Grading of Glial Tumours: A Prospective Study

机译：免疫组织化学标志物P53和KI-67在胶质肿瘤分级中的作用：前瞻性研究

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Gliomas are the tumours of neuroepithelial tissues and are named according to their cell of origin.Squash cytology and sterotactic biopsies with Haematoxylin and Eosin (H&E) staining form the backbone of diagnosis, nevertheless recent advances in Immunohistochemistry (IHC) have revolutionised the way gliomas are diagnosed and graded.Aim: To evaluate expression and correlation of p53 and Ki-67/ MIB-1 amongst a series of gliomas diagnosed morphologically according to World Health Organisation (WHO) classification of Central Nervous System (CNS) tumours 2007.Materials and Methods: The present prospective study was conducted over a period of 18 months from December 2016 to May 2018 at a tertiary care centre in Uttarakhand, India.The study group comprised all consecutive cases of glial tumours that were clinically diagnosed and histopathologically confirmed as Gliomas during this period.Histopathological sections were made from formalin fixed tissue and stained with H&E and grading was done according to the WHO grading system 2007 for CNS neoplasms.Subsequently, IHC sections were taken on poly L-lysine coated slides and IHC staining of p53 and MIB-1 (Ki-67) was performed.The IHC scores were calculated and correlated with histopathological grade.Statistical analysis was done using Statistical Package for the Social Sciences (SPSS) software version 17.0.Results: A total of 40 cases of glial tumours diagnosed on histopathology were included in the study.They showed M:F ratio of 1.22:1 with peak age incidence of 21-30 years.Astrocytoma, grade IV was the most frequent diagnosis followed by grade II on H&E.On IHC, grade II diffuse astrocytomas, grade III Anaplastic Astrocytomas (AA), and Glioblastoma Multiforme (GBM) demonstrated a mean p53 positivity of 29.5, 50.83 and 47.66, respectively and a Ki-67 positivity of 32.5, 48.33 and 58.08, respectively.For ependymomas grade I, II and III, the mean p53 positivity was 9, 5.26 and 12, respectively and the mean Ki-67 positivity was 2, 7.93 and 40, respectively.Amongst oligodendrogliomas grade II and III showed a p53 positivity of 6 and 8.25 and Ki-67 positivity of 12 and 30.5, respectively.An association was found between an increase in histologic grade and proliferation markers.Conclusion: Histologic grade was the most important prognostic factor with respect to patient survival in glial neoplasms.The immunopositivity for p53 and Ki-67 correlated well with histological malignancy grade in all glioma subtypes, but a considerable overlap of proliferative index was observed between different subtypes.
机译：胶质瘤是神经上皮组织的肿瘤，并根据其用苏木精和曙红（H＆安培; E）origin.Squash细胞学和立体定向活检的细胞被命名为染色形式诊断的支柱，但最近在免疫组织化学（IHC）的进步已经彻底改变了/ MIB-1之间的一系列神经胶质瘤的形态确诊根据世界卫生组织中枢神经系统（CNS）的（WHO）分类肿瘤2007.Materials评价表达和p53的相关性和Ki-67：神经胶质瘤诊断和graded.Aim方法：本前瞻性研究在北阿坎德邦三级保健中心在18个月内进行，从2016年12月2018年5月，India.The研究组包括神经胶质肿瘤的所有连续的案例，临床诊断和病理证实为脑胶质瘤在此period.Histopathological切片从福尔马林固定的组织制备和用H＆amp; E染色和分级根据WHO等级系统2007用于CNS neoplasms.Subsequently做，IHC切片采取在聚L-赖氨酸包被的载玻片和IHC染色p53和的MIB-1（Ki-67的）为performed.The IHC分数计算和相关与组织病理学grade.Statistical分析使用统计软件包社会科学进行（SPSS）软件版本17.0.Results：共40例确诊病理组织学神经胶质肿瘤的被列入study.They表明L：1.22 F比： 1 21-30 years.Astrocytoma的峰值年龄发病，IV级是最常见的诊断随后II级上H＆安培; E.On的IHC，等级II漫星形细胞瘤，级间变性III星形细胞瘤（AA），以及胶质母细胞瘤（GBM ）证明为29.5，分别50.83 47.66和，平均值的p53阳性和32.5，48.33和58.08的Ki-67阳性，室管膜瘤respectively.For级I，II和III中，平均的p53阳性为9，分别5.26和12，和平均Ki-67的POS itivity为2，7.93和40，respectively.Amongst少突级II和III显示12和30.5的6和8.25和Ki-67阳性的p53的阳性，respectively.An相关性增加组织学分级和增殖标记物之间发现。结论：组织学等级是相对于患者的生存在胶质neoplasms.The免疫阳性中p53和Ki-67与组织学恶性程度在所有胶质瘤亚型密切相关的最重要的预后因素，但不同亚型之间观察到的增殖指数的一个相当大的重叠物。

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