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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Evaluation of Truenat MTB/RIF Test in Comparison with Microscopy and Culture for Diagnosis of Extrapulmonary Tuberculosis in a Tertiary Care Centre
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Evaluation of Truenat MTB/RIF Test in Comparison with Microscopy and Culture for Diagnosis of Extrapulmonary Tuberculosis in a Tertiary Care Centre

机译：与初学中心诊断术造影细胞瘤诊断的术

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Worldwide, Extrapulmonary Tuberculosis (EPTB) accounts for 15-20% of all cases of TB.The diagnosis of EPTB is a big challenge, as the number of Mycobacterium tuberculosis (MTB) bacilli in the tissues and other organs is often very low.Truenat MTB/RIF (rifampicin) is a novel method, which is battery operated, point-of-care and chip-based Real Time Polymerase Chain Reaction (RT-PCR) micro device.Aim: To evaluate Truenat as a screening test in the diagnosis of EPTB in comparison with microscopy and culture.Materials and Methods: A prospective cross-sectional study was carried out over a year in which samples from suspected cases of EPTB fitting in the inclusion criteria were subjected to ZiehlNeelsen (ZN) staining for smear microscopy, culture on Lowenstein Jensen (LJ) medium and PCR for MTB by Truenat.Comparisons were made between the tests and the data was presented using summary statistics with 95% Confidence Interval (CI).Results: A total of 248 samples were received from suspected cases of EPTB.Out of the different samples tested, 9 (3.6%) were positive with Truenat MTB.The predominant type of EPTB observed in the study was lymph node Tuberculosis (TB) (66.6%) followed by intestinal, pleural and skeletal TB.Out of the 106 samples tested for culture, four were culture positive for MTB and out of 178 samples tested for microscopy, three were positive for acid fast bacilli.Sensitivity, specificity, Negative Predictive Value (NPV), Positive Predictive Value (PPV), observed agreement of Truenat with culture and microscopy were 100%, 95.1%, 100%, 44.4%, 95.3% and 100%, 96.6%, 100%, 33.3%, 96.6%, respectively.Conclusion: Truenat MTB test is a cost-effective rapid molecular test with good sensitivity and specificity for the diagnosis of EPTB in low resource settings.
机译：在世界范围内，肺外结核（EPTB）占的EPTB的TB.The诊断的所有病例的15-20％是一个很大的挑战，如结核分枝杆菌（MTB）是在组织和其他器官杆菌往往很数目low.Truenat MTB / RIF（利福平）是一种新的方法，它是电池操作的，点的护理和基于芯片的实时聚合酶链式反应（RT-PCR）的微device.Aim：评价Truenat作为诊断的筛选试验EPTB的与显微镜和culture.Materials和方法比较：前瞻性横断面研究用，其中来自在纳入标准拟合EPTB疑似病例样品进行ZiehlNeelsen（ZN）染色涂片镜检一年进行， A的248个样本总被从怀疑CA接收：由测试和数据之间作了Truenat.Comparisons的Lowenstein詹森（LJ）介质和PCR对MTB上的培养物使用具有95％置信区间（CI）。结果汇总统计呈现测试，9（3.6％）的不同样品的EPTB.Out的SES在该研究中具有阳性Truenat MTB.The主要类型EPTB的观察是淋巴结结核随后肠，胸膜和骨骼TB（TB）（66.6％）用于培养测试的106个样本的.out，四个是培养阳性MTB和出于对显微镜测试178克的样品，3为阳性抗酸bacilli.Sensitivity，特异性，阴性预测值（NPV），阳性预测值（PPV）与培养和显微镜检查的Truenat观察协议分别为100％，95.1％，100％，44.4％，95.3％和100％，96.6％，100％，33.3％，96.6％。结论：Truenat MTB测试是一种成本 - 有效快速分子试验具有良好的敏感性和特异性的EPTB在低资源设置诊断。

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