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首页> 外文期刊>Chemical Science International Journal >Evaluation of Secondary Metabolites and Antioxidant Activity of Water, Ethyl Acetate and Hexane Fractions from the Mangrove Young Leaves Sonneratia alba
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Evaluation of Secondary Metabolites and Antioxidant Activity of Water, Ethyl Acetate and Hexane Fractions from the Mangrove Young Leaves Sonneratia alba

机译：从红树林幼树的次级代谢物和抗氧化剂活性评价红树林幼叶Sonneratia Alba的水，乙酸乙酯和己烷级分

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The fractions from young leaves of mangrove Sonneratia alba was studied for its associated secondary metabolites and antioxidant activities. The objective of this study was to determine the secondary metabolite components and antioxidant activity of water, ethyl acetate, and hexane fractions of the young leaves of mangrove S. alba. The fraction was obtained from dry powder of young leaf S.alba using continuous fractionation of crude extracts. The crude extract was attained by 2 extraction methods (soxhlet and maceration) and 2 extraction solvents (methanol and ethanol). Secondary metabolites analyses were qualitatively conducted to detect the presence or absence of phenols, flavonoid, tannin, steroid, triterpenoid and alkaloid. Total phenols were measured using Folin Ciocalteau reagents and gallic acid standard curves whereas antioxidant activity were analyzed using DPPH method (1- 1-diphenil-2-picrihydrasil). Results showed that all fractions contained secondary metabolite components tested. The highest rendement was found in the water fraction fromsoxhletation extract with methanol (6.36±0.29%). The total phenol values were found the highest in the ethylacetatefraction from macerated extract with ethanol (352±9.77 mgGAE/g). Stronger antioxidant activity was also found in ethylacetate? fraction as indicated by the small value of IC50 DPPH namely the ethylacetate fraction with soxhletation extract with ethanol? (3.43±0.25 μg / mL). The results of this study indicate that the semipolar fraction (ethylacetate fraction) has more potential as a source of natural antioxidants.
机译：研究了红树郡Sonneratia Alba的年轻叶子的馏分，为其相关的次生代谢物和抗氧化活性研究。本研究的目的是确定红树林的年轻叶片的次级代谢物成分和水，乙酸乙酯和己烷部分的抗氧化活性。使用粗提取物的连续分馏从幼叶S.Alba的干粉中获得级分。通过2萃取方法（SoxHlet和浸渍）和2个萃取溶剂（甲醇和乙醇）获得粗提物。次级代谢产物分析是定性进行的，以检测酚，类黄酮，单宁，类固醇，三萜类化合物和生物碱的存在或不存在。使用Folin Ciocalteau试剂和Gallic酸标准曲线测量总酚，而使用DPPH方法分析抗氧化活性（1-1-二维硅-2- PICRIHYDRASIL）。结果表明，所有级分含有测试的次级代谢物成分。在用甲醇（6.36±0.29％）中，在水分馏分中发现最高酵素（6.36±0.29％）。发现总酚值在用乙醇的浸渍萃取液中溶于乙基乙酸溶液中最高（352±9.77 mggae / g）。在乙酸乙酯中也发现较强的抗氧化活性？通过IC50 dpph的少量值表示的级分，即用乙醇与索氏提取物的乙基乙酸馏分馏分？ （3.43±0.25μg/ ml）。该研究的结果表明，半极性级分（乙酸乙酯级分）具有更潜力作为天然抗氧化剂的来源。

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