• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>CHROMATOGRAPHY >Multi-Functional Nanocavities Fabricated Using Molecular Imprinting and Post-Imprinting Modifications for Efficient Biomarker Detection
【24h】

Multi-Functional Nanocavities Fabricated Using Molecular Imprinting and Post-Imprinting Modifications for Efficient Biomarker Detection

机译：使用分子印迹制造的多功能纳米胶质，以及用于高效生物标志物检测的印迹后改性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Antibodies and enzymes are currently considered the gold-standard molecular recognition elements as they facilitate the construction of biosensing systems and exhibit high specificity and affinity toward target molecules. However, the low stability of such systems and high associated production cost limit the practical applications of antibodies and enzymes, thereby necessitating the development of alternative molecular recognition elements. Molecularly imprinted polymers (MIPs) are synthetic polymer receptors that are capable of molecular recognition. These polymers contain binding cavities of various shapes and sizes that are complementary to the target molecule and aid in the capture of target molecules. However, although the original procedure for generating MIPs, developed before 2000, is simple, the resulting binding activity and selectivity are inferior to those of antibodies. Meanwhile, post-imprinting modification (PIM) involves site-directed chemical modification of functional monomer residues within the molecularly imprinted cavities to alter MIP functionality. In this review, we provide an overview of sophisticated PIM techniques for developing highly sensitive MIPs that can be used to recognize biomarker proteins. Toward this, we draw heavily on information from our own recent work. This article has the potential to provide important insights that would aid the development of synthetic polymer materials for biosensing.
机译：抗体和酶目前被认为是金标准的分子识别元件，因为它们有助于构建生物传感系统并对靶分子表现出高特异性和亲和力。然而，这种系统的低稳定性和高相关的生产成本限制了抗体和酶的实际应用，从而需要替代分子识别元件的发展。分子印迹聚合物（MIPS）是能够分子识别的合成聚合物受体。这些聚合物含有与靶分子互补的各种形状和尺寸的结合腔，并有助于捕获靶分子。然而，尽管在2000年之前发育了MIP的原始程序很简单，但是由此产生的结合活性和选择性差不等于抗体的结合活性。同时，印迹后改性（PIM）涉及分子印迹腔内的功能单体残留物的现场导向化学改性，以改变MIP官能度。在本次审查中，我们提供了用于开发可用于识别生物标志物蛋白的高度敏感的MIPS的复杂PIM技术概述。对此，我们从我们最近的工作中大量获取信息。本文有可能提供重要的见解，以帮助开发用于生物传感的合成聚合物材料。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号