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首页> 外文期刊>Journal of genetics >Whole mitogenome analysis and phylogeny of freshwater fish red-finned catopra (Pristolepis rubripinnis) endemic to Kerala, India
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Whole mitogenome analysis and phylogeny of freshwater fish red-finned catopra (Pristolepis rubripinnis) endemic to Kerala, India

机译:淡水鱼红翅片豆荚(Pristolopis Rubripinnis)对印度喀拉拉邦的整体促型分析和系统发育

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The freshwater leaf fish Pristolepis rubripinnis belongs to the family Pristolepididae, restricted to Pamba and Chalakudy rivers of Kerala, India. In the present study, we sequenced the complete mitogenome of P. rubripinnis and analysed its phylogeny in the order Anabantiformes. The 16622-bp long genome comprised of 13 protein-coding genes, two rRNA genes, 22 transfer RNAs (tRNAs) genes and had a noncoding control region. All the protein-coding genes, tRNA and rRNA were located on the heavy strand, except nad6 and eighttRNAs (glutamine, alanine, asparagine, cysteine, tyrosine, serine, glutamic acid and proline) transcribed from L strand. The genome exhibited an overlapping between atp8 and atp6 (2 bp), nad4 and nad4l (2 bp), tRNAIle and tRNAGln (1 bp), tRNAThr and tRNAPro (1 bp).Around 157 bp, an intergenic spacer was identified. The overall GC-skews and AT-skews of the H-strand mitogenome were –0.35 and 0.079, respectively, revealing that the H-strand consisted of equal amounts of A and T and that the overall nucleotide composition was Cskewed. All tRNA genes exhibited cloverleaf secondary structures, while the secondary structure of tRNASer lacked a discernible dihydrouridine stem. The phylogenetic analysis of available mitogenomes of Anabantiformes revealed a sister group relationship between Pristolepididae and Channidae. The whole mitogenome of Pristolepis rubripinnis will form a molecular resource for further taxonomic and conservation studies on this endemic freshwater fish.
机译:淡水叶鱼饲养葡萄球菌葡萄干葡萄球菌属于家庭原药哌啶,仅限于印度喀拉拉邦的PAMBA和Chalakudy河流。在本研究中,我们测序了P. Rubripinnis的完整促催化剂,并在Anabantiforms顺序中分析了其系统发育。 16622-BP长基因组由13个蛋白质编码基因,两个rRNA基因,22个转移RNA(TrNAS)基因并具有非编码对照区域。所有蛋白质编码基因,TRNA和RRNA位于重股,除了NAD6和八克拉斯(谷氨酰胺,丙氨酸,天冬酰胺,半胱氨酸,酪氨酸,丝氨酸,谷氨酸和脯氨酸)以外的L股。该基因组在ATP8和ATP6(2bp),NAD4和NAD4L(2bp)之间具有重叠,TRNAILE和TRNAGLN(1bp),TRNATHR和TRNAPRO(1bp).Around 157bp,鉴定了代际间隔物。 H-链介质组的总GC-Skews和偏差分别为-0.35和0.079,揭示了由相等量A和T组成的H股,并且整个核苷酸组合物被Cskewed。所有TRNA基因都表现出克拉夫次级结构,而Trnaser的二级结构缺少可辨别的二氢鲁红茎。 Anabantiformes可用诱导毒蛛的系统发育分析显示了原粒哌啶和通道之间的姐妹群关系。 Pristolopis Rubripinnis的整个促使催化剂将形成该流动淡水鱼的其他分类和保护研究的分子资源。

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