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首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >Relatedness of type IV pilin PilA amongst geographically diverse Moraxella bovoculi isolated from cattle with infectious bovine keratoconjunctivitis
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Relatedness of type IV pilin PilA amongst geographically diverse Moraxella bovoculi isolated from cattle with infectious bovine keratoconjunctivitis

机译：IV型Pilin Pila的相关性在地理上不同的<斜视> moraxella Bovoculi 从牛与传染性牛角膜炎神经炎

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Introduction Moraxella bovoculi is frequently isolated from the eyes of cattle with infectious bovine keratoconjunctivitis (IBK; pinkeye). As with M. bovis, which has been causally linked to IBK, M. bovoculi expresses an RTX (repeats in the structural toxin) cytotoxin that is related to M. bovis cytotoxin. Pilin, another pathogenic factor in M. bovis , is required for corneal attachment. Seven antigenically distinct pilin serogroups have been described in M. bovis . Hypothesis/Gap Statement Multiple different serogroups exist amongst type IV pilin encoded by M. bovis , however, it is not known whether M. bovoculi exhibits a similar degree of diversity in type IV pilin that it encodes. Aim This study was done to characterize a structural pilin (PilA) encoded by M. bovoculi isolated from cases of IBK to determine if diversity exists amongst PilA sequences. Methodology Ninety-four isolates of M. bovoculi collected between 2002 and 2017 from 23 counties throughout California and from five counties in four other Western states were evaluated. Results DNA sequencing and determination of deduced amino acid sequences revealed ten (designated groups A through J) unique PilA sequences that were ~96.1–99.3?%?identical. Pilin groups A and C matched previously reported putative PilA sequences from M. bovoculi isolated from IBK-affected cattle in the USA (Virginia, Nebraska, and Kansas) and Asia (Kazakhstan). The ten pilin sequences identified were only ~74–76?%?identical to deduced amino acid sequences of putative pilin proteins identified from the previously reported whole-genome sequences of M. bovoculi derived from deep nasopharyngeal swabs of IBK-asymptomatic cattle. Conclusions Compared to the diversity reported between structural pilin proteins amongst different serogroups of M. bovis , M. bovoculi PilA from geographically diverse isolates derived from IBK-affected cattle are more conserved.
机译：引言Moraxella Bovoculi经常与牛的眼睛与传染性牛角膜炎（IBK; Pinkeye）孤立。与副植物有因果关系到IBK，M.Bovoculi表达了与M. Bovis细胞毒素相关的细胞毒素的RTX（在结构毒素中重复）。角膜附着需要Pilin，牛肉杉的另一种致病因子。已经在M. Bovis中描述了七种抗原性不同的菌株血清群组。假设/间隙声明在M. Bovis编码的IV型菌本中存在多个不同的血清组，然而，尚不清楚M. Bovoculi是否表现出它编码的IV型Pilin中的类似程度的多样性。目的本研究已经完成，以表征由从IBK病例中分离的M.Bovoculi编码的结构pilin（Pila），以确定是否存在Pila序列中的多样性。从加利福尼亚州的23个县和四个其他西方国家的23个县中收集的M.Bovovuli的九十四分离株M. Bovoculi的含量九十四个分离株。结果DNA测序和推导的氨基酸序列的测定揭示了十（指定的组A至J）独特的皮拉序列，其〜96.1-99.3≤％？相同。 Pilin Groups A和C匹配从美国IBK受影响的牛群中的M.Bovoculi匹配的先前报告的PUTAP PILA序列（弗吉尼亚州，内布拉斯加和堪萨斯州）和亚洲（哈萨克斯坦）。鉴定的10个菌株序列仅为〜74-76？％？与先前报道的衍生自IBK-无论牛的深鼻咽拭子的M.Bovophari的全基因组序列中鉴定的推定菌蛋白的推导率氨基酸序列相同。结论与结构Plin蛋白在不同血清中的不同血清中报告的多样性相比，来自来自IBK受影响的牛的地理位置分离物的M.Bovoculi Pla更加守恒。

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