...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >Immunomodulatory streptococci that inhibit CXCL8 secretion and NFκB activation are common members of the oral microbiota
【24h】

Immunomodulatory streptococci that inhibit CXCL8 secretion and NFκB activation are common members of the oral microbiota

机译：抑制CXCL8分泌和NFκB活化的免疫调节链球菌是口服微生物群的常见成员

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction Oral tissues are generally homeostatic despite exposure to many potential inflammatory agents including the resident microbiota. This requires the balancing of inflammation by regulatory mechanisms and/or anti-inflammatory commensal bacteria. Thus, the levels of anti-inflammatory commensal bacteria in resident populations may be critical in maintaining this homeostatic balance. Hypothesis/Gap Statement The incidence of immunosuppressive streptococci in the oral cavity is not well established. Determining the proportion of these organisms and the mechanisms involved may help to understand host-microbe homeostasis and inform development of probiotics or prebiotics in the maintenance of oral health. Aim To determine the incidence and potential modes of action of immunosuppressive capacity in resident oral streptococci. Methodology Supragingival plaque was collected from five healthy participants and supragingival and subgingival plaque from five with gingivitis. Twenty streptococci from each sample were co-cultured with epithelial cells±flagellin?or LL-37. CXCL8 secretion was detected by ELISA, induction of cytotoxicity in human epithelial cells by lactate dehydrogenase release and NFκB-activation using a reporter cell line. Bacterial identification was achieved through partial 16S rRNA gene sequencing and next-generation sequencing. Results CXCL8 secretion was inhibited by 94/300 isolates. Immunosuppressive isolates were detected in supragingival plaque from healthy (4/5) and gingivitis (4/5) samples, and in 2/5 subgingival (gingivitis) plaque samples. Most were Streptococcus mitis/oralis . Seventeen representative immunosuppressive isolates all inhibited NFκB activation. The immunosuppressive mechanism was strain specific, often mediated by ultra-violet light-labile factors, whilst bacterial viability was essential in certain species. Conclusion Many streptococci isolated from plaque suppressed epithelial cell CXCL8 secretion, via inhibition of NFκB. This phenomenon may play an important role in oral host-microbe homeostasis.
机译：介绍口腔组织通常是稳态，尽管暴露于包括驻留微生物群的许多潜在炎症剂。这需要通过调节机制和/或抗炎的非抗菌细菌进行炎症的平衡。因此，居民群体中的抗炎性聚苯菌细菌的水平对于维持这种稳态平衡可能是至关重要的。假设/间隙陈述口腔中免疫抑制链球菌的发生率不是很好的。确定这些生物的比例和所涉及的机制可能有助于了解宿主微生物宿舍，并在维持口腔健康方面提供益生菌或益生元的发展。目的是确定常驻口腔链球菌免疫抑制能力的发病率和潜在作用方式。方法过度凝视斑块从五个健康参与者和Supricalival和HiNgivivencival和龈斑块中收集。从上皮细胞±鞭毛蛋白α或LL-37共培养20个来自每个样品的链球菌。通过ELISA检测CXCL8分泌，通过使用报告细胞系通过乳酸脱氢酶释放和NFκB活化诱导人上皮细胞中细胞毒性的诱导。通过部分16SRRNA基因测序和下一代测序来实现细菌鉴定。结果CXCL8分泌抑制94/300分离物。从健康（4/5）和牙龈炎（4/5）样品中，在Supriticallum斑块中检测免疫抑制分离物，并在2/5龈下（牙龈炎）斑块样品中。大多数是链球菌/ oralis。 17个代表性免疫抑制性分离株全部抑制NFκB活化。免疫抑制机制是特异性的菌​​株，经常被紫外线稳定因子介导，而细菌活力在某些物种中是必需的。结论许多从斑块抑制上皮细胞CXCL8分泌中分离的链球菌，通过抑制NFκB。这种现象可能在口服宿主微生物宿舍中起重要作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号