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首页> 外文期刊>Journal of Clinical Microbiology >Self-Collected Saline Gargle Samples as an Alternative to Health Care Worker-Collected Nasopharyngeal Swabs for COVID-19 Diagnosis in Outpatients
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Self-Collected Saline Gargle Samples as an Alternative to Health Care Worker-Collected Nasopharyngeal Swabs for COVID-19 Diagnosis in Outpatients

机译：自收集的盐水漱口样品作为医疗保健工作者收集的鼻咽拭子的替代品，用于住院患者的Covid-19诊断

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We assessed the performance, stability, and user acceptability of swab-independent self-collected saliva and saline mouth rinse/gargle sample types for the molecular detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in adults and school-aged children. Outpatients who had recently been diagnosed with COVID-19 or were presenting with suspected COVID-19 were asked to have a nasopharyngeal (NP) swab collected and provide at least one self-collected sample type. ABSTRACT We assessed the performance, stability, and user acceptability of swab-independent self-collected saliva and saline mouth rinse/gargle sample types for the molecular detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in adults and school-aged children. Outpatients who had recently been diagnosed with COVID-19 or were presenting with suspected COVID-19 were asked to have a nasopharyngeal (NP) swab collected and provide at least one self-collected sample type. Participants were also asked about sample acceptability using a five-point Likert scale. For those previously diagnosed with COVID-19, all samples underwent real-time PCR testing using a lab-developed assay, and the majority were also tested using an FDA-authorized assay. For those presenting with suspected COVID-19, only those with a positive nasopharyngeal swab sample went on to have other samples tested. Saline mouth rinse/gargle and saliva samples were tested daily at time zero, day 1, and day 2 to assess nucleic acid stability at room temperature. Fifty participants (aged 4 to 71?years) were included; of these, 40 had at least one positive sample and were included in the primary sample yield analysis. Saline mouth rinse/gargle samples had a sensitivity of 98% (39/40), while saliva samples had a sensitivity of 79% (26/33). Both saline mouth rinse/gargle and saliva samples showed stable viral RNA detection after 2?days of room temperature storage. Mouth rinse/gargle samples had the highest (mean, 4.9) and health care worker (HCW)-collected NP swabs had the lowest acceptability scores (mean, 3.1). In conclusion, saline mouth rinse/gargle samples demonstrated higher combined user acceptability ratings and analytical performance than saliva and HCW-collected NP swabs. This sample type is a promising swab-independent option, particularly for outpatient self-collection in adults and school-aged children.
机译：我们评估的性能，稳定性和用户棉签独立的自我收集唾液和生理盐水漱口水/含漱液样本类型的可接受性严重急性呼吸综合征冠状病毒2（SARS-COV-2）的成人和学龄儿童的分子检测孩子们。谁最近被诊断出患有COVID-19或疑似COVID-19展示了门诊被要求收集鼻咽（NP）拭子和提供至少一个自我收集的样品类型。摘要我们评估的性能，稳定性，和用户拭子独立自收集唾液和盐水漱口/漱口样品类型的可接受用于成人和分子检测严重急性呼吸综合征冠状病毒2（SARS-CoV的-2）的学校为适龄儿童。谁最近被诊断出患有COVID-19或疑似COVID-19展示了门诊被要求收集鼻咽（NP）拭子和提供至少一个自我收集的样品类型。与会者还被问及使用了五点李克特量表样品合格。对于先前诊断有COVID-19的，所有的样品进行使用实验室开发的检测实时PCR检测，大部分都使用FDA授权的检测也检测。对于那些怀疑COVID-19呈现，只有那些具有正鼻咽拭子样品继续有其它样品进行测试。盐水漱口水/漱口和唾液样品在时间零每日测试，第1天，第2天，以评估在室温下核酸的稳定性。五十参与者（？4岁〜71岁）被列入;的这些，40有至少一个正样品并包括在原始样品产量分析。盐水漱口/漱口样品具有98％（39/40）灵敏度，而唾液样品具有79％（26/33）灵敏度。两个盐水漱口水/漱口和唾液样品显示后的室温下储存2？天稳定病毒RNA的检测。漱口水/含漱液样本具有最高（平均4.9）和卫生保健工作者（HCW）-collected NP拭子的最低可接受分数（平均3.1）。总之，盐水漱口水/表现出较高的组合的用户可接受性评级和比唾液和HCW收集的NP拭子分析性能漱口样本。这种样本类型是一个有前途的棉签无关的选项，尤其是对于门诊自我收集的成人和学龄儿童。

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