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首页> 外文期刊>American Journal of Translational Research >miR-214-5p targeted by LncRNA DANCR mediates TGF-β signaling pathway to accelerate proliferation, migration and inhibit apoptosis of prostate cancer cells
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miR-214-5p targeted by LncRNA DANCR mediates TGF-β signaling pathway to accelerate proliferation, migration and inhibit apoptosis of prostate cancer cells

机译：MiR-214-5P靶向LNCRNA DANCR介导TGF-β信号传导途径，以加速增殖，迁移和抑制前列腺癌细胞的凋亡

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Objective: This research was designed to probe into the regulatory mechanism of long non-coding RNA (LncRNA) differentiation antagonizing non-protein coding RNA (DANCR) in potential applications and molecular mechanisms of prostate carcinoma (PC). Methods: The DANCR and miR-214-5p levels in PC tissues and cell lines were tested via real-time PCR, and those of transforming growth factor-β (TGF-β) signaling pathway related proteins were evaluated via Western Blot (WB). Cell proliferation, migration, apoptosis and the regulatory relationship between target genes were assessed via MTT method, scratch test, flow cytometry, dual-luciferase report, RNA co-immunoprecipitation and RNA pull-down test, respectively. Results: DANCR was up-regulated in PC patients’ serum and cell lines, while miR-214-5p was opposite, showing negative correlation. Besides, DANCR was significantly correlated with PSA, Gleason score and T stage in PC patients. The area under the curve (AUC) of DANCR and miR-214-5p for diagnosing PC was not less than 0.850, while the AUC for predicting poor prognosis was more than 0.800. Cox analysis results also revealed that the two might be prognostic indicators of PC patients. We found that DANCR high levels or miR-214-5p low levels were related to PC patients’ poor prognosis. Up-regulating DANCR or down-regulating miR-214-5p could promote PC cells’ malignant proliferation and migration, prevent apoptosis, and activate TGF-β signaling pathway, while reverse treatment of DANCR or miR-214-5p can reverse the above results. DANCR regulates miR-214-5p in a targeted manner, and DANCR over-expression can reduce the cancer inhibitory effect of miR-214-5p on PC cells. Conclusion: DANCR-miR-214-5p-TGF-β axis regulatory network plays a key regulatory part in PC progression. It may provide new strategies for the screening and treatment of patients.
机译：目的：该研究旨在探讨在前列腺癌（PC）的潜在应用和分子机制中的长非编码RNA（LNCRNA）分化拮抗非蛋白质编码RNA（DANCR）的调节机制。方法：通过实时PCR测试PC组织和细胞系中的DANCR和MIR-214-5P水平，通过Western印迹（WB）评估转化生长因子-β（TGF-β）信号传导途径相关蛋白质的方法。通过MTT方法，划痕试验，流式细胞术，双荧光素酶报告，RNA共灌注和RNA下拉试验评估靶基因之间的细胞增殖，迁移，细胞凋亡和调节关系。结果：DANCR在PC患者的血清和细胞系中上调，而MIR-214-5P相反，显示出负相关。此外，DANCR与PSA，GLEASES评分和PC患者T阶段显着相关。 DANCR和MIR-214-5P的曲线（AUC）下的区域不小于0.850，而预测预测差的AUC则大于0.800。 COX分析结果还显示，这两者可能是PC患者的预后指标。我们发现DANCR高水平或MIR-214-5P低水平与PC患者的预后有关。调节丹麦或下调miR-214-5p可以促进PC细胞的恶性增殖和迁移，预防细胞凋亡，并激活TGF-β信号通路，而丹麦克或MIR-214-5P的反向治疗可以逆转上述结果。 DANCR以目标方式调节miR-214-5p，丹麦克过度表达可以降低PC细胞miR-214-5p的癌症抑制作用。结论：DANCR-MIR-214-5P-TGF-β轴监管网络在PC进展中播放了一个关键的监管部位。它可能为患者的筛查和治疗提供新的策略。

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